FTIR-determination of sterols from the red alga Asparagopsis armata: Comparative studies with HPLC

An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase car...

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Bibliographic Details
Published in:Talanta (Oxford) 2006-02, Vol.68 (4), p.1230-1235
Main Authors: El Hattab, Naïma, Daghbouche, Yasmina, Hattab, Mohamed El, Piovetti, Louis, Garrigues, Salvador, Guardia, Miguel de la
Format: Article
Language:English
Subjects:
Analytical chemistry
Asparagopsis armata
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Fourier transform infrared
High performance liquid chromatography
Other chromatographic methods
Red alga
Solid phase extraction
Spectrometric and optical methods
Sterol determination
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Summary:An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase cartridges (SPE) and eluted with dichloromethane stabilized with β-amylene. Fourier transform infrared (FTIR) spectrometry was employed for selective detection at 1049 cm −1 with a baseline established between 1000 and 1079 cm −1. The results were compared to those obtained by high performance liquid chromatography (HPLC). The concentration obtained in actual samples from alga was 3.37% (w/w) by FTIR and 3.30% (w/w) by HPLC, showing a good comparability between the two methods.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2005.07.023