Determination of proline in wine using flow injection analysis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection

• Published in: Talanta (Oxford) 2004-11, Vol.64 (4), p.894-898
• Main Authors: Costin, Jason W., Barnett, Neil W., Lewis, Simon W.
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Chemical and thermal methods; Chemiluminescence; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Flow injection analysis; Other chromatographic methods; Proline; Ru(bipy) 33; Tris(2,2′-bipyridyl)ruthenium(II); Wine
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2004.03.065

Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10 −6 M) and the detection limit was 1 × 10 −8 M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy) 3 3+ consumption and its associated effect on accuracy.