Tris(2,2′-bipyridyl)ruthenium(II)–Zirconia–Nafion composite modified electrode applied as solid-state electrochemiluminescence detector on electrophoretic microchip for detection of pharmaceuticals of tramadol, lidocaine and ofloxacin

• Published in: Talanta (Oxford) 2006-10, Vol.70 (3), p.572-577
• Main Authors: Ding, Shou-Nian, Xu, Jing-Juan, Zhang, Wen-Jia, Chen, Hong-Yuan
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Lidocaine; Microchip; Ofloxacin; Other chromatographic methods; Solid-state electrochemiluminescence; Tramadol; Tris(2,2′-bipyridyl)ruthenium(II); Zirconia–Nafion composite film
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2006.01.017

Tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+)–Zirconia–Nafion composite modified glassy carbon disk electrode as a solid-state electrochemiluminescence (ECL) detector is successfully applied to an electrophoretic microchip system with a wall-jet configuration. Pharmaceuticals such as tramadol, lidocaine and ofloxacin were selected to characterize the performance of this microchip capillary electrophoresis (CE)–ECL detection system. Voltammetric and ECL behaviors of immobilized Ru(bpy) 3 2+ were investigated in lidocaine system. Influences of the separation electric field to cyclic voltammograms (CVs) of the immobilized Ru(bpy) 3 2+ were also investigated. Tramadol, lidocaine and ofloxacin can be baseline separated without any additives. The detection limits (S/N = 3) were 2.5 × 10 −5 mol L −1 for tramadol, 5.0 × 10 −6 mol L −1 for lidocaine, 1.0 × 10 −5 mol L −1 for ofloxacin under the sample injection of picoliters, and the linear ranges were from 5.0 × 10 −5 to 2.5 × 10 −3 mol L −1 for tramadol, 1.0 × 10 −5 to 1.0 × 10 −3 mol L −1 for lidocaine, and 1.0 × 10 −5 to 2.5 × 10 −3 mol L −1 for ofloxacin, respectively.