Isolation and characterization of pathogenic Leishmania turanica from Nesokia indica (Rodentia, Muridae) by PCR-RFLP and ITS1 sequencing in Iran

Zoonotic cutaneous leishmaniasis, primarily an infection of desert rodents, is an important endemic disease in some parts of Iran. In spite of reporting Leishmania infection in Nesokia indica microscopically , its role remains unclear in Iran. We report the first natural infection by Leishmania tura...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 2009-11, Vol.103 (11), p.1177-1179
Main Authors: Hajjaran, H., Mohebali, M., Alimoradi, S., Abaei, M.R., Edrissian, Gh.H.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cricetinae
DNA, Recombinant - genetics
General aspects
Iran
Leishmania
Leishmania - genetics
Leishmania - isolation & purification
Leishmania turanica
Leishmaniasis, Cutaneous - parasitology
Medical sciences
Mesocricetus
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Muridae
Nesokia indica
Pathogenicity
PCR-RFLP
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Rats
Rodentia
Sequence Analysis, DNA
Species Specificity
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Summary:Zoonotic cutaneous leishmaniasis, primarily an infection of desert rodents, is an important endemic disease in some parts of Iran. In spite of reporting Leishmania infection in Nesokia indica microscopically , its role remains unclear in Iran. We report the first natural infection by Leishmania turanica in a wild-caught N. indica in the west part of Iran, near the border with Iraq. The evidence is in the form of PCR-RFLP testing based on internal transcribed spacer 1 (ITS1) rDNA with HaeIII enzyme and ITS1 sequence analysis. This isolate was highly pathogenic in BALB/c mice and golden hamsters and caused lethal systemic infection in the former.
ISSN:0035-9203
1878-3503
DOI:10.1016/j.trstmh.2008.08.016