Development and characterization of polymorphic microsatellite markers in Linum usitatissimum

Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, an...

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Bibliographic Details
Published in:Journal of plant research 2010-01, Vol.123 (1), p.119-123
Main Authors: Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops, Long, S, He, D, Li, X, Wang, Y, Liu, J, Chen X
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
Biomarkers
Biomedical and Life Sciences
Botany
Cultivars
Dinucleotide Repeats - genetics
Flax - genetics
GENE
Gene Frequency - genetics
Gene mapping
GENES
GENETIC MARKERS
Genome, Plant - genetics
Genomics
http://www.fao.org/aos/agrovoc#c_24030
http://www.fao.org/aos/agrovoc#c_27583
http://www.fao.org/aos/agrovoc#c_3214
http://www.fao.org/aos/agrovoc#c_36574
http://www.fao.org/aos/agrovoc#c_4355
http://www.fao.org/aos/agrovoc#c_6088
http://www.fao.org/aos/agrovoc#c_8157
Life Sciences
LINUM USITATISSIMUM
MARCADORES GENETICOS
MARQUEUR GENETIQUE
MICROSATELITES
MICROSATELLITE
Microsatellite Repeats - genetics
MICROSATELLITES
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Plant Biochemistry
Plant Ecology
Plant Physiology
Plant Sciences
POLIMORFISMO
POLYMORPHISM
Polymorphism, Genetic - genetics
POLYMORPHISME
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Technical Note
VARIEDADES
VARIETE
VARIETIES
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Description
Summary:Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-009-0271-3