Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: a proof-of-concept study

Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and n...

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Published in:Journal of clinical periodontology 2010-01, Vol.37 (1), p.9-16
Main Authors: Salvi, Giovanni E., Franco, Lea M., Braun, Thomas M., Lee, Angie, Rutger Persson, Gösta, Lang, Niklaus P., Giannobile, William V.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Bacteria - classification
Biofilms
biomarker
Biomarkers - analysis
Case-Control Studies
Cohort Studies
Dental Plaque - microbiology
Dental Plaque Index
Dentistry
diabetes
Diabetes Mellitus, Type 1 - complications
Diabetes Mellitus, Type 1 - metabolism
Follow-Up Studies
gingival crevicular fluid
Gingival Crevicular Fluid - chemistry
gingivitis
Gingivitis - metabolism
Humans
IL-1β
Inflammation Mediators - analysis
Interleukin-1beta - analysis
Interleukin-8 - analysis
Matrix Metalloproteinase 8 - analysis
Matrix Metalloproteinase 9 - analysis
MMP
Nucleic Acid Hybridization
Periodontal Index
Prospective Studies
Young Adult
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Summary:Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL‐1β, IL‐8, MMP‐8 and MMP‐9 were quantified by enzyme‐linked immunosorbent assay, and SP samples were assessed by DNA–DNA hybridization for a panel of 40 subgingival microbial species. Results: IL‐1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7–21 days while healthy patients showed IL‐1β increases from baseline to 14–21 days (p
ISSN:0303-6979
1600-051X
DOI:10.1111/j.1600-051X.2009.01500.x