The Expression Analysis of Periostin in Human Breast Cancer

Background To investigate the expression of periostin in breast cancer and its clinical relevance. Methods Periostin mRNA expression in breast tissues was measured by RT-PCR. Periostin protein in breast tissues was evaluated by Western blot. Immunohistochemistry method (Envision method) was carried...

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Bibliographic Details
Published in:The Journal of surgical research 2010-05, Vol.160 (1), p.102-106
Main Authors: Zhang, Yanan, M.D, Zhang, Guangyuan, M.D, Li, Junsheng, M.D, Tao, Qingsong, M.D, Tang, Wenhao, M.D
Format: Article
Language:English
Subjects:
Adult
Aged
Biomarkers, Tumor - metabolism
breast cancer
Breast Neoplasms - metabolism
Carcinoma, Ductal, Breast - metabolism
Cell Adhesion Molecules - metabolism
cell growth
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Middle Aged
oncogene
periostin
RNA, Messenger - metabolism
Surgery
Up-Regulation
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Summary:Background To investigate the expression of periostin in breast cancer and its clinical relevance. Methods Periostin mRNA expression in breast tissues was measured by RT-PCR. Periostin protein in breast tissues was evaluated by Western blot. Immunohistochemistry method (Envision method) was carried out to detect the expression of periostin in breast cancer tissues of different clinical stages and metastasized lymph nodes, as well as benign breast disease. Results The expression of periostin in cancer tissue was increased at both mRNA level ( P < 0.05) and protein level ( P < 0.05) compared with benign and normal breast tissues. The periostin protein expression level increased with breast cancer clinical stages as shown by IHC. Conclusion Periostin was overexpressed in breast cancer tissues as opposed to normal breast, which implies its role in the pathogenesis and development of this disorder.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2008.12.042