Protein kinase C-δ is involved in induction of NOX1 gene expression by aldosterone in rat vascular smooth muscle cells

In this study, we focused on the relationship between aldosterone and NOX1 expression in vascular smooth muscle cells (VSMCs). For the first time, with the use of specific inhibitors of protein kinase C (PKC), we report that PKCδ mediates upregulation of NOX1 induced by 10 nM aldosterone in cultured...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2010-03, Vol.75 (3), p.304-309
Main Authors: Wei, Haiyan, Mi, Xuhua, Ji, Ling, Yang, Lichuan, Xia, Qingjie, Wei, Yuquan, Miyamori, Isamu, Fan, ChunYuan
Format: Article
Language:English
Subjects:
Aldosterone
Aldosterone - pharmacology
Animals
Base Sequence
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Corticosteroids
DNA Primers
Gene expression
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Enzymologic - physiology
Gene Silencing
Genes
Genetic engineering
Genetic research
Life Sciences
Microbiology
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
NADH, NADPH Oxidoreductases - genetics
NADPH Oxidase 1
Protein biosynthesis
Protein Kinase C-delta - genetics
Protein Kinase C-delta - physiology
Protein kinases
Rats
RNA
Smooth muscle
Steroids
Superoxide
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Summary:In this study, we focused on the relationship between aldosterone and NOX1 expression in vascular smooth muscle cells (VSMCs). For the first time, with the use of specific inhibitors of protein kinase C (PKC), we report that PKCδ mediates upregulation of NOX1 induced by 10 nM aldosterone in cultured VSMCs. Participation of PKC in the mediation of NOX1 regulation was further confirmed by the effect of diacylglycerol, a PKC agonist, on the NOX1 RNA in A7r5 cells with Northern blot analysis. To establish cause and effect, we next silenced the PKCδ gene partly by RNA interference and found knockdown of PKCδ gene attenuated aldosterone-induced NOX1 expression, generation of superoxide, as well as protein synthesis in VSMCs. Taken together, these data indicated PKCδ might mediate aldosterone-dependent NOX1 upregulation in VSMCs. In addition, we showed that the cascade from aldosterone to PKCδ activation had the participation of the mineralocorticoid receptor.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297910030065