Allergen IgE-isotype-specific suppression by maternally derived monoclonal anti-IgG-idiotype

• Published in: Allergy (Copenhagen) 2010, Vol.65 (1), p.16-23
• Main Authors: Tanasa, R.I, Trad, A, Lange, H, Grötzinger, J, Lemke, H
• Format: Article
• Language: English
• Subjects: Allergens - immunology; Allergies; allergy; Amino Acid Sequence; Animals; Antibodies, Anti-Idiotypic - chemistry; Antibodies, Anti-Idiotypic - immunology; Bee Venoms - immunology; Biological and medical sciences; Dermatology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; hypersensitivity; Hypersensitivity - immunology; IgE suppression; Immunization; Immunoglobulin E - immunology; Immunoglobulin G - immunology; Immunoglobulin Idiotypes; Immunoglobulins; maternal anti-idiotype; maternal antibodies; Maternal Exposure; Medical sciences; Mice; Mice, Inbred CBA; Models, Molecular; Molecular Sequence Data; Monoclonal antibodies; Phospholipases A2 - immunology; Pregnancy; Protein Conformation; Rodents; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; transgenerational memory
• ISSN: 0105-4538; 1398-9995
• DOI: 10.1111/j.1398-9995.2009.02104.x

The dramatic increase of IgE-mediated allergic diseases in western countries demonstrates the urgent need for new therapeutic or prophylactic approaches. In mice, a prophylactic long-lasting allergen-specific suppression of IgE responsiveness is induced by maternal IgG antibodies to allergens like ovalbumin, phospholipase A₂ (bvPLA₂) or ovomucoid. As neonatal application or maternally derived pathogen-reactive antibodies (idiotypes) as well as corresponding anti-idiotypes can induce anti-microbial protection, we probed the transgenerational IgE-suppressive mechanism with a syngeneic monoclonal anti-idiotypic antibody. The monoclonal bee-venom-phospholipase A₂ (bvPLA₂)-reactive IgG antibody MS613 (idiotype) or the corresponding syngeneic anti-idiotype II/2-19 were injected during the first 2 days postpartum to the dams. Immunization of offspring with minute doses of IgE-inducing bvPLA₂ was started at an adult age of 3[fraction one-half] months. The postnatal transfer of the anti-bvPLA₂ idiotype MS613 or the corresponding anti-idiotype II/2-19 induced long-lasting allergen-specific IgE suppression in a dose-dependent manner, while the IgG response to the allergen developed normally. Quantitatively, the anti-idiotype was more effective than idiotype. Molecular modeling of the idiotype-anti-idiotype complex and its comparison with the bvPLA₂ structure revealed that the anti-idiotype does not mimic bvPLA₂ epitopes and thus can not be regarded as an internal image antibody and, consequently, does not function as a surrogate antigen. Idiotypic network reactivity is at least one major factor for induction of transgenerational IgE suppression by maternal IgG antibodies. If applicable to humans, these data suggest the possibility of a prophylactic and possibly therapeutic treatment of IgE-mediated allergic diseases with anti-idiotypic antibodies.