Lippia origanoides chemotype differentiation based on essential oil GC-MS and principal component analysis

Chromatographic (GC/flame ionization detection, GC/MS) and statistical analyses were applied to the study of essential oils and extracts obtained from flowers, leaves, and stems of Lippia origanoides plants, growing wild in different Colombian regions. Retention indices, mass spectra, and standard s...

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Bibliographic Details
Published in:Journal of separation science 2010, Vol.33 (1), p.93-103
Main Authors: Stashenko, Elena E, Martínez, Jairo R, Ruíz, Carlos A, Arias, Ginna, Durán, Camilo, Salgar, William, Cala, Mónica
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Colombia
Cosmetics
Essential oil
Essential oils
Extraction plants
Gas Chromatography-Mass Spectrometry - methods
GC/MS
Leaves
Lippia - chemistry
Lippia origanoides
Oils, Volatile - analysis
Pinocembrin
Plant Oils - analysis
Plants (organisms)
Principal component analysis
Principal Component Analysis - methods
Stems
Thymol
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Summary:Chromatographic (GC/flame ionization detection, GC/MS) and statistical analyses were applied to the study of essential oils and extracts obtained from flowers, leaves, and stems of Lippia origanoides plants, growing wild in different Colombian regions. Retention indices, mass spectra, and standard substances were used in the identification of 139 substances detected in these essential oils and extracts. Principal component analysis allowed L. origanoides classification into three chemotypes, characterized according to their essential oil major components. α- and β-phellandrenes, p-cymene, and limonene distinguished chemotype A; carvacrol and thymol were the distinctive major components of chemotypes B and C, respectively. Pinocembrin (5,7-dihydroxyflavanone) was found in L. origanoides chemotype A supercritical fluid (CO₂) extract at a concentration of 0.83±0.03 mg/g of dry plant material, which makes this plant an interesting source of an important bioactive flavanone with diverse potential applications in cosmetic, food, and pharmaceutical products.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900452