Efficacy of the Bm86 antigen against immature instars and adults of the dog tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae)

The Bm86 antigen has been used to control ticks of the Boophilus genera in integrated programs that also include the use of acaricides. Because of recent phylogenetic studies have lead to the inclusion of all Boophilus species within the Rhipicephalus genera, we aimed to investigate the efficacy of...

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Bibliographic Details
Published in:Veterinary parasitology 2010-02, Vol.167 (2-4), p.321-326
Main Authors: Perez-Perez, D., Bechara, G.H., Machado, R.Z., Andrade, G.M., del Vecchio, R.E.M., Pedroso, M.S., Hernández, M.V., Farnós, O.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Bm86
Dog
Dog Diseases - blood
Dog Diseases - parasitology
Dog Diseases - prevention & control
Dogs
Female
Larva
Male
Membrane Glycoproteins - immunology
Nymph
Recombinant protein
Recombinant Proteins - immunology
Rhipicephalus - immunology
Rhipicephalus sanguineus
Tick Infestations - prevention & control
Vaccine
Vaccines - immunology
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Summary:The Bm86 antigen has been used to control ticks of the Boophilus genera in integrated programs that also include the use of acaricides. Because of recent phylogenetic studies have lead to the inclusion of all Boophilus species within the Rhipicephalus genera, we aimed to investigate the efficacy of the Bm86 antigen on the biotic potential of Rhipicephalus sanguineus. Domestic dogs were vaccinated with Bm86 and challenged with the three instars of R. sanguineus. Male and female mongrel dogs were divided into two groups of four animals each, comprising non-vaccinated and vaccinated animals. Immunized dogs were given two doses of an experimental formulation containing 50μg of recombinant Bm86, at 21 days interval while the other group was given placebo, consisting of the same preparation without Bm86. Each dog was challenged 21 days after the last dose with 250 larvae, 100 nymphs and 55 adults (25 females and 30 males) released inside feeding chambers (one per instar) glued to their shaved flank. The effect of the vaccination was evaluated by determining biological parameters of ticks including the yield rates of larvae, nymphs and adult females. Adult females engorged weight, egg mass weight, efficiency rate of conversion to eggs (ERCE) and hatchability. In addition, sera were collected from dogs at 0, 21, 36, 45 and 75 days after the vaccination and used for the detection of specific antibodies by ELISA. Collection rates of larvae, nymphs and adult females fed on vaccinated dogs were significantly (p
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2009.09.034