Clinical Observation on the Endocrinal and Immune Functions in Subjects with Yin-deficiency Constitution

• Published in: Chinese journal of integrative medicine 2010-02, Vol.16 (1), p.28-32
• Main Author: 王琦 任小娟 姚实林 吴宏东
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers - analysis; Biomarkers - blood; Endocrine System - metabolism; Endocrine System - physiopathology; Female; Humans; Hypothalamo-Hypophyseal System - physiopathology; Immune System - metabolism; Immune System - physiopathology; Male; Medicine; Medicine & Public Health; Medicine, Chinese Traditional - methods; Middle Aged; Nucleosides - blood; Observation; Original Article; Pituitary-Adrenal System - physiopathology; Thyroid Gland - physiopathology; Yin Deficiency - blood; Yin Deficiency - diagnosis; Yin Deficiency - immunology; Yin Deficiency - physiopathology; Young Adult; 临床观察; 促肾上腺皮质激素; 免疫功能; 内分泌; 夹心ELISA; 白细胞介素-1β; 白细胞介素1β
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-010-0028-9

Objective： To explore the relationship between yin-deficiency constitution （YDC） and biochemical indexes by way of observing the endocrinal and immune functions in subjects with YDC. Methods： On the basis of epidemiological investigation, 60 subjects with YDC and 50 with gentle constitution （GC） were selected according to the pertinent criteria. From each subject, 8 mL of fasting venous blood was drawn at 8：00-9：00 in the morning, with the serum separated by centrifugation 3 000 r/min for 5 min and preserved at -70℃ in a freezer. Serum levels of corticosterone, cortisol, adrenocorticotrophic hormone （ACTH）, cyclic adenosine monophosphate （cAMP）, cyclic guanosine monophosphate （cGMP）, free triiodothyronine （FT3）, free thyroxine （1=-1-4）, throtropic stimulation hormone, interleukin 1β （IL-1β ） and interleukin 2 （IL-2） were detected by doubleantibody sandwich ELISA; cAMP/cGMP ratio was calculated, and the difference between the two constitutions in terms of these indexes was analyzed. Results： Serum FT3 was 4.16 ± 1.38 pmol/L in subjects with YDC, which was higher than that in subjects with GC （3.71 ±0.55 pmol/L）, but levels of cortisol （124.58± 45.36 ng/mL）, ACTH （58.92± 14.55 pg/mL）, cGMP （66.00 ± 18.02 pmol/mL） and FT4 （12.33 ± 3.12 pmol/L） in YDC were lower than those in GC （13.43±2.31 pmol/L）, showing significant difference （P〈0.05）. Conclusion： YDC is related to some extent with the disturbances in the hypothalamus-pituitary-adrenal axis, hypothalamus-pituitary-thyroid axis, cyclic nucleoside system and immune function.