Embroidered and Surface Modified Polycaprolactone-Co-Lactide Scaffolds as Bone Substitute: In Vitro Characterization

The aim of this study was to evaluate an embroidered polycaprolactone-co-lactide (trade name PCL) scaffold for the application in bone tissue engineering. The surface of the PCL scaffolds was hydrolyzed with NaOH and coated with collagen I (coll I) and chondroitin sulfate (CS). It was investigated i...

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Bibliographic Details
Published in:Annals of biomedical engineering 2009-10, Vol.37 (10), p.2118-2128
Main Authors: Rentsch, Barbe, Hofmann, Andre, Breier, Annette, Rentsch, Claudia, Scharnweber, Dieter
Format: Article
Language:English
Subjects:
Adult
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedicine
Biophysics
Bone Substitutes - chemistry
Cell Differentiation
Cells, Cultured
Classical Mechanics
Female
Hardness
Humans
Hydrophobic and Hydrophilic Interactions
Male
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Polyesters - chemistry
Polymers
Pore size
Porosity
Sodium hydroxide
Stem cells
Surface Properties
Tissue Engineering - methods
Young Adult
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Summary:The aim of this study was to evaluate an embroidered polycaprolactone-co-lactide (trade name PCL) scaffold for the application in bone tissue engineering. The surface of the PCL scaffolds was hydrolyzed with NaOH and coated with collagen I (coll I) and chondroitin sulfate (CS). It was investigated if a change of the surface properties and the application of coll I and CS could promote cell adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSC). The porosity (80%) and pore size (0.2-1 mm) of the scaffold could be controlled by embroidery technique and should be suitable for bone ingrowth. The treatment with NaOH made the polymer surface more hydrophilic (water contact angle dropped to 25%), enhanced the coll I adsorption (up to 15%) and the cell attachment (two times). The coll I coated scaffold improved cell attachment and proliferation (three times). CS, as part of the artificial matrix, could induce the osteogenic differentiation of hMSC without other differentiation additives. The investigated scaffolds could act not just as temporary matrix for cell migration, proliferation, and differentiation in bone tissue engineering but also have a great potential as bioartificial bone substitute.
ISSN:0090-6964
1573-9686
DOI:10.1007/s10439-009-9731-0