Protecting effects of a large dose of dexamethasone on spleen injury of rats with severe acute pancreatitis

• Published in: Journal of gastroenterology and hepatology 2010-02, Vol.25 (2), p.302-308
• Main Authors: Xiping, Zhang, Ruiping, Zhang, Binyan, Yu, Li, Zhou, Hanqing, Chen, Wei, Zhu, Rongchao, Ying, Jing, Ye, Wenqin, Yuan, Jinjin, Bai
• Format: Article
• Language: English
• Subjects: Acute Disease; Animals; Anti-Inflammatory Agents - administration & dosage; apoptosis; Apoptosis - drug effects; bcl-2-Associated X Protein - metabolism; Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; dexamethasone; Dexamethasone - administration & dosage; Disease Models, Animal; Endothelin-1 - blood; Endotoxins - blood; Gastroenterology. Liver. Pancreas. Abdomen; Inflammation Mediators - blood; Injuries of the abdomen. Foreign bodies of the digestive system; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Nitric Oxide - blood; Other diseases. Semiology; Pancreatitis - chemically induced; Pancreatitis - complications; Pancreatitis - drug therapy; Pancreatitis - metabolism; Pancreatitis - pathology; Pharmacology. Drug treatments; Phospholipases A2 - blood; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rats, Sprague-Dawley; severe acute pancreatitis; Severity of Illness Index; spleen; Spleen - drug effects; Spleen - metabolism; Spleen - pathology; Splenic Diseases - etiology; Splenic Diseases - metabolism; Splenic Diseases - pathology; Splenic Diseases - prevention & control; Taurocholic Acid; Time Factors; tissue microarrays; Traumas. Diseases due to physical agents
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1111/j.1440-1746.2009.05999.x

Background and Aims:  To explore the protecting effects and mechanisms of dexamethasone on spleen injury in rats with severe acute pancreatitis (SAP). Methods:  The rats were randomly divided into a model control group, treated group and sham‐operated group. The contents of plasma endotoxin, serum NO, phospholipase A2 enzyme (PLA2) and endothelin‐1 (ET‐1) were determined. The mortality rate, pathological changes and changes of Bax and Bcl‐2 protein expression levels and apoptotic indexes in the spleen of rats were observed in all groups, respectively, at 3, 6 and 12 h after operation. Results:  Although the survival rate was significantly higher in the treated group than in the model control group, there was no significantly different between them (P > 0.05). The expression levels of Bax and Bcl‐2 proteins and apoptotic indexes were significantly higher in the treated group than in the model control group at different time points (P