Investigation of Porcine Endogenous Retrovirus in the Conservation Population of Ningxiang Pig

Abstract Porcine endogenous retrovirus (PERV) varies between pig breeds. Screening and analysis of PERV in putative pig breeds may provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. In this study, PERV was investigated among the conservation popula...

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Bibliographic Details
Published in:Transplantation proceedings 2009-12, Vol.41 (10), p.4389-4393
Main Authors: Xing, X.W, Hawthorne, W.J, Yi, S, Simond, D.M, Dong, Q, Ye, B, Tong, Q.J, Ye, Z, Wang, W
Format: Article
Language:English
Subjects:
Animals
China
DNA Primers
DNA, Mitochondrial - genetics
DNA, Viral - blood
DNA, Viral - genetics
DNA, Viral - isolation & purification
Endogenous Retroviruses - genetics
Endogenous Retroviruses - physiology
Gammaretrovirus
Genetic Variation
Humans
Organ Preservation - methods
Organ Preservation - standards
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Viral - blood
RNA, Viral - genetics
RNA, Viral - isolation & purification
RNA-Directed DNA Polymerase
Species Specificity
Surgery
Swine - blood
Swine - genetics
Swine - virology
Transcription, Genetic
Transplantation, Heterologous - trends
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Summary:Abstract Porcine endogenous retrovirus (PERV) varies between pig breeds. Screening and analysis of PERV in putative pig breeds may provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. In this study, PERV was investigated among the conservation population of the Ningxiang pig. The result revealed that the genotype of PERV distribution was subtype A, 100%; subtype B, 100%; and subtype C, 100%. The env sequences of PERV-A and -B showed 11 clones detected by Kpn I and Mbo I digestion, indicating that there existed multiple variants of PERV-A and -B in the Ningxiang pig. Reverse transcriptase polymerase chain reaction results showed that PERV had transcriptional activity in these individuals. In addition, PERV A/C recombinant was detected in most individuals of Ningxiang pig. Because PERV A/C recombinants increase the potential infectious risk, the breed may not be a proper donor for xenotransplantation.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2009.09.051