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Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing
The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition...
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| Published in: | Veterinary microbiology 2010-05, Vol.142 (3-4), p.193-198 |
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| cites | cdi_FETCH-LOGICAL-c415t-cffdc2d5c0c3bca033cdc2787db198a2ec3f25a062a5f2a4dd9ceb8dbf9752483 |
| container_end_page | 198 |
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| container_title | Veterinary microbiology |
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| creator | Brinkhof, J.M.A. Houwers, D.J. Moll, L. Dercksen, D. van Maanen, C. |
| description | The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.
The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of |
| doi_str_mv | 10.1016/j.vetmic.2009.09.060 |
| format | article |
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The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of <3% within-herd prevalence using 1/10 pre-diluted samples and even <1% within-herd prevalence when samples were tested undiluted. In a panel of 249 bulk milk samples, all samples from SRLV free flocks (n=138) tested negative in the ELISA, while 50% of the samples from flocks with an unknown SRLV-status (n=111) were positive. For a subset of 59 bulk milk samples, agreement between ELISA results and leader-gag PCR results was almost 100%. These results demonstrate the potential of bulk milk testing as a cost effective tool for early detection of infection in dairy flocks, which is essential for SRLV-monitoring programs.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2009.09.060</identifier><identifier>PMID: 19913363</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>(Bulk) milk ; accuracy ; animal diseases ; Animals ; antibodies ; Antibodies, Viral - analysis ; Antibodies, Viral - blood ; Arthritis-Encephalitis Virus, Caprine - physiology ; Biological and medical sciences ; blood plasma ; CAEV ; diagnostic techniques ; disease diagnosis ; disease prevalence ; disease surveillance ; DNA ; ELISA ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - veterinary ; ewe milk ; Fundamental and applied biological sciences. Psychology ; Goat Diseases - diagnosis ; goat milk ; Goats ; immune response ; Lentivirus ; Lentivirus Infections - diagnosis ; Lentivirus Infections - veterinary ; Limit of Detection ; Maedi visna virus ; microbial genetics ; Microbiology ; Milk - chemistry ; Milk - immunology ; PCR ; Plasma ; Pneumonia, Progressive Interstitial, of Sheep - diagnosis ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; Population Surveillance - methods ; proviruses ; Reproducibility of Results ; RNA, Viral - analysis ; Sheep ; Sheep Diseases - diagnosis ; small ruminant lentiviruses ; species differences ; SRLV ; vertebrate viruses ; viral diseases of animals and humans ; Visna-maedi virus - physiology</subject><ispartof>Veterinary microbiology, 2010-05, Vol.142 (3-4), p.193-198</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-cffdc2d5c0c3bca033cdc2787db198a2ec3f25a062a5f2a4dd9ceb8dbf9752483</citedby><cites>FETCH-LOGICAL-c415t-cffdc2d5c0c3bca033cdc2787db198a2ec3f25a062a5f2a4dd9ceb8dbf9752483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22753540$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19913363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brinkhof, J.M.A.</creatorcontrib><creatorcontrib>Houwers, D.J.</creatorcontrib><creatorcontrib>Moll, L.</creatorcontrib><creatorcontrib>Dercksen, D.</creatorcontrib><creatorcontrib>van Maanen, C.</creatorcontrib><title>Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.
The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of <3% within-herd prevalence using 1/10 pre-diluted samples and even <1% within-herd prevalence when samples were tested undiluted. In a panel of 249 bulk milk samples, all samples from SRLV free flocks (n=138) tested negative in the ELISA, while 50% of the samples from flocks with an unknown SRLV-status (n=111) were positive. For a subset of 59 bulk milk samples, agreement between ELISA results and leader-gag PCR results was almost 100%. These results demonstrate the potential of bulk milk testing as a cost effective tool for early detection of infection in dairy flocks, which is essential for SRLV-monitoring programs.</description><subject>(Bulk) milk</subject><subject>accuracy</subject><subject>animal diseases</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Viral - analysis</subject><subject>Antibodies, Viral - blood</subject><subject>Arthritis-Encephalitis Virus, Caprine - physiology</subject><subject>Biological and medical sciences</subject><subject>blood plasma</subject><subject>CAEV</subject><subject>diagnostic techniques</subject><subject>disease diagnosis</subject><subject>disease prevalence</subject><subject>disease surveillance</subject><subject>DNA</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>ewe milk</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Goat Diseases - diagnosis</subject><subject>goat milk</subject><subject>Goats</subject><subject>immune response</subject><subject>Lentivirus</subject><subject>Lentivirus Infections - diagnosis</subject><subject>Lentivirus Infections - veterinary</subject><subject>Limit of Detection</subject><subject>Maedi visna virus</subject><subject>microbial genetics</subject><subject>Microbiology</subject><subject>Milk - chemistry</subject><subject>Milk - immunology</subject><subject>PCR</subject><subject>Plasma</subject><subject>Pneumonia, Progressive Interstitial, of Sheep - diagnosis</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Population Surveillance - methods</subject><subject>proviruses</subject><subject>Reproducibility of Results</subject><subject>RNA, Viral - analysis</subject><subject>Sheep</subject><subject>Sheep Diseases - diagnosis</subject><subject>small ruminant lentiviruses</subject><subject>species differences</subject><subject>SRLV</subject><subject>vertebrate viruses</subject><subject>viral diseases of animals and humans</subject><subject>Visna-maedi virus - physiology</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kduKFDEQhoMo7rj6BqK5EW_sMYc-3iws46oLA8qO621IJ5WezHZ32qR7YZ7Q1zI9PeidUBAq9dVfRf0IvaZkTQnNPx7WjzB2Vq0ZIdV6jpw8QStaFjxhWcqeohXhRZlQyrML9CKEAyEkrXLyHF3QqqKc53yFfn-ysuldGK3CA3jjfCd7BdgZfLO93V1j2Wv8fXOHbY-thn605mj7Bu_utj8T2xtQI2gc9gDDCW2cHAOewswE8FP3AQ-tDJ08VTvbPuAgu6GFcPqIqiB9e8QaxihlXT9PXnTnJNa1tP6ITevUQ8Dj3rup2eN6ikIntRHi7n3zEj0zsg3w6vxeovvPNz82X5Ptty-3m-ttolKajYkyRiumM0UUr5UknKuYF2Wha1qVkoHihmWS5ExmhslU60pBXeraVEXG0pJfoveL7uDdrynOFp0NCtpW9uCmIArOq2hPUUUyXUjlXQgejBi87aQ_CkrE7KA4iMVBMTso5shJbHtzHjDVHeh_TWfLIvDuDMigZGt89MuGvxxjRcazdBZ6u3BGOiEbH5n7HSOUE1rylJ82vFoIiAd7tOBFUBai-9r6eH-hnf3_rn8Am-HJMw</recordid><startdate>20100519</startdate><enddate>20100519</enddate><creator>Brinkhof, J.M.A.</creator><creator>Houwers, D.J.</creator><creator>Moll, L.</creator><creator>Dercksen, D.</creator><creator>van Maanen, C.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100519</creationdate><title>Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing</title><author>Brinkhof, J.M.A. ; Houwers, D.J. ; Moll, L. ; Dercksen, D. ; van Maanen, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-cffdc2d5c0c3bca033cdc2787db198a2ec3f25a062a5f2a4dd9ceb8dbf9752483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>(Bulk) milk</topic><topic>accuracy</topic><topic>animal diseases</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Viral - analysis</topic><topic>Antibodies, Viral - blood</topic><topic>Arthritis-Encephalitis Virus, Caprine - physiology</topic><topic>Biological and medical sciences</topic><topic>blood plasma</topic><topic>CAEV</topic><topic>diagnostic techniques</topic><topic>disease diagnosis</topic><topic>disease prevalence</topic><topic>disease surveillance</topic><topic>DNA</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>ewe milk</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Goat Diseases - diagnosis</topic><topic>goat milk</topic><topic>Goats</topic><topic>immune response</topic><topic>Lentivirus</topic><topic>Lentivirus Infections - diagnosis</topic><topic>Lentivirus Infections - veterinary</topic><topic>Limit of Detection</topic><topic>Maedi visna virus</topic><topic>microbial genetics</topic><topic>Microbiology</topic><topic>Milk - chemistry</topic><topic>Milk - immunology</topic><topic>PCR</topic><topic>Plasma</topic><topic>Pneumonia, Progressive Interstitial, of Sheep - diagnosis</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Population Surveillance - methods</topic><topic>proviruses</topic><topic>Reproducibility of Results</topic><topic>RNA, Viral - analysis</topic><topic>Sheep</topic><topic>Sheep Diseases - diagnosis</topic><topic>small ruminant lentiviruses</topic><topic>species differences</topic><topic>SRLV</topic><topic>vertebrate viruses</topic><topic>viral diseases of animals and humans</topic><topic>Visna-maedi virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brinkhof, J.M.A.</creatorcontrib><creatorcontrib>Houwers, D.J.</creatorcontrib><creatorcontrib>Moll, L.</creatorcontrib><creatorcontrib>Dercksen, D.</creatorcontrib><creatorcontrib>van Maanen, C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brinkhof, J.M.A.</au><au>Houwers, D.J.</au><au>Moll, L.</au><au>Dercksen, D.</au><au>van Maanen, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2010-05-19</date><risdate>2010</risdate><volume>142</volume><issue>3-4</issue><spage>193</spage><epage>198</epage><pages>193-198</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.
The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of <3% within-herd prevalence using 1/10 pre-diluted samples and even <1% within-herd prevalence when samples were tested undiluted. In a panel of 249 bulk milk samples, all samples from SRLV free flocks (n=138) tested negative in the ELISA, while 50% of the samples from flocks with an unknown SRLV-status (n=111) were positive. For a subset of 59 bulk milk samples, agreement between ELISA results and leader-gag PCR results was almost 100%. These results demonstrate the potential of bulk milk testing as a cost effective tool for early detection of infection in dairy flocks, which is essential for SRLV-monitoring programs.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19913363</pmid><doi>10.1016/j.vetmic.2009.09.060</doi><tpages>6</tpages></addata></record> |
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| subjects | (Bulk) milk accuracy animal diseases Animals antibodies Antibodies, Viral - analysis Antibodies, Viral - blood Arthritis-Encephalitis Virus, Caprine - physiology Biological and medical sciences blood plasma CAEV diagnostic techniques disease diagnosis disease prevalence disease surveillance DNA ELISA enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - veterinary ewe milk Fundamental and applied biological sciences. Psychology Goat Diseases - diagnosis goat milk Goats immune response Lentivirus Lentivirus Infections - diagnosis Lentivirus Infections - veterinary Limit of Detection Maedi visna virus microbial genetics Microbiology Milk - chemistry Milk - immunology PCR Plasma Pneumonia, Progressive Interstitial, of Sheep - diagnosis polymerase chain reaction Polymerase Chain Reaction - veterinary Population Surveillance - methods proviruses Reproducibility of Results RNA, Viral - analysis Sheep Sheep Diseases - diagnosis small ruminant lentiviruses species differences SRLV vertebrate viruses viral diseases of animals and humans Visna-maedi virus - physiology |
| title | Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing |
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