Gold Nanoparticle-Fluorophore Complexes: Sensitive and Discerning "Noses" for Biosystems Sensing

Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn‐on is observed. By judicious choice of the functionalized NP and the fluorophore, these complexes display different responses to analytes, thus leading to versatile y...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2010-04, Vol.49 (19), p.3268-3279
Main Authors: Bunz, Uwe H. F., Rotello, Vincent M.
Format: Article
Language:English
Subjects:
Animals
Bacteria - isolation & purification
Biosensing Techniques
biosensors
Diphosphates - chemistry
displacement assays
Fluorescent Dyes - chemistry
fluorescent probes
Gold - chemistry
Humans
Metal Nanoparticles - chemistry
Mice
Microarray Analysis
nanoparticles
Polymers - chemistry
Proteins - chemistry
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Summary:Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn‐on is observed. By judicious choice of the functionalized NP and the fluorophore, these complexes display different responses to analytes, thus leading to versatile yet simple array‐based sensor platforms. Using this strategy, we can identify proteins in buffer and serum, distinguish between both different species and different strains of bacteria, and differentiate between healthy, cancerous, and metastatic human and murine cells. Disruption desired: Different monolayer‐protected nanoparticle–fluorophore constructs are used in indicator‐displacement assays to spy on proteins, bacteria, cells, and ions (see picture). The modus operandi involves disruption of the preformed quencher–fluorophore complexes, leading to partial and analyte‐dependent fluorescence turn‐on. Small libraries of nanoparticle–fluorophore complexes get the sensing job for different biological analytes done.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200906928