Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical cord-blood derived CD34+ cells

Previous work has shown that optimal ex vivo expansion and differentiation of CD34+ progenitor cells into neutrophils is by addition of Flt3‐L, SCF and G‐CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated u...

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Bibliographic Details
Published in:Vox sanguinis 2010-04, Vol.98 (3p1), p.e290-e294
Main Authors: Gallagher, R. C. J., Tura-Ceide, O., Turner, M., Barclay, R.
Format: Article
Language:English
Subjects:
Antigens, CD34 - analysis
Blood
CD34+ stem cell
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cells, Cultured - cytology
Cells, Cultured - metabolism
Cellular biology
differentiation
DNA, Complementary - genetics
Fetal Blood - cytology
Frizzled Receptors - genetics
Gene expression
Gene Expression Profiling
Hematopoietic Cell Growth Factors - pharmacology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - metabolism
Humans
Infant, Newborn
Leukocytes
neutrophil
Neutrophils - cytology
Polymerase Chain Reaction - methods
qPCR
RNA, Messenger - genetics
Signal transduction
Transcription, Genetic
WNT genes
Wnt Proteins - genetics
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Summary:Previous work has shown that optimal ex vivo expansion and differentiation of CD34+ progenitor cells into neutrophils is by addition of Flt3‐L, SCF and G‐CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34+ cells, however statistically significant changes in gene expression are not always consistent across UCB samples.
ISSN:0042-9007
1423-0410
DOI:10.1111/j.1423-0410.2009.01299.x