Isolation, Amino-Acid Sequence, Synthesis and Biological Properties of Urotensin I from Hippoglossoides elassodon

• Published in: Journal of neuroendocrinology 1990-12, Vol.2 (6), p.875-882
• Main Authors: McMaster, D., Itoh, H., MacCannell, K. L., Rivier, J., Rivier, C., Vale, W., Fryer, J. N., Tran, T. N., Lederis, K.
• Format: Article
• Language: English
• Subjects: adrenocorticotropin release; amino-acid sequence; Hippoglossoides elassodon; urotensin I; vasodilatation
• ISSN: 0953-8194; 1365-2826
• DOI: 10.1111/j.1365-2826.1990.tb00654.x

A 41‐residue urotensin I neuropeptide (H‐UI) was isolated from urophyses of the marine teleost Hippoglossoides elassodon (the flathead sole). The peptide was recognized by its partial cross‐reactivity in a radioimmunoassay developed for Catostomus (sucker) Ul (S‐UI), and was purified by reversed‐phase high‐performance liquid chromatography. The amino‐acid sequence was shown to be H‐Ser‐Glu‐Glu‐Pro‐Pro‐Met‐Ser‐lle‐Asp‐Leu‐Thr‐Phe‐His‐Met‐Leu‐Arg‐Asn‐Met‐lle‐His‐Arg‐Ala‐Lys‐Met‐Glu‐Gly‐Glu‐Arg‐Glu‐Gln‐Ala‐Leu‐lle‐Asn‐Arg‐Asn‐Leu‐Leu‐Asp‐Glu‐Val‐NH2. H‐UI is 66% homologous with S‐UI and 63% homologous with Cyprinus (carp) Ul (C‐UI). Like S‐ and C‐UI, H‐UI is about 50% homologous with the frog skin peptide sauvagine and with Catostomus and mammalian corticotropin‐releasing factors. H‐UI had similar vasodilatory effects in mammals, and similar adrenocorticotropin‐releasing effects (in rat and goldfish) to S‐UI, C‐UI, sauvagine and the corticotropin‐releasing factors, but had relatively low potency (e.g. 10% to 30% of the vasodilatory potency of S‐ and C‐UI) in all the bioassay systems studied.