Retinal transplantation of neural progenitor cells derived from the brain of GFP transgenic mice

Neural progenitor cells isolated from the brains of neonatal GFP transgenic mice were grafted to the retina of RCS rats and rds and B6 mice. Expression of GFP and differentiation markers was evaluated at 1–4 weeks post-transplantation. Grafted cells maintained transgene expression throughout the 4-w...

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Bibliographic Details
Published in:Vision research (Oxford) 2003-07, Vol.43 (16), p.1699-1708
Main Authors: Mizumoto, Hiroyuki, Mizumoto, Keiko, Shatos, Marie A, Klassen, Henry, Young, Michael J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - cytology
Cell Differentiation
Cell Line
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
GFP transgenic mice
Graft Survival
Green fluorescent protein
Green Fluorescent Proteins
Indicators and Reagents - analysis
Luminescent Proteins - analysis
Mice
Mice, Inbred BALB C
Neural progenitor cells
Rats
Retina - cytology
Retinal transplantation
Stem Cell Transplantation
Stem Cells - cytology
Transplantation, Heterologous
Vertebrates: nervous system and sense organs
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Summary:Neural progenitor cells isolated from the brains of neonatal GFP transgenic mice were grafted to the retina of RCS rats and rds and B6 mice. Expression of GFP and differentiation markers was evaluated at 1–4 weeks post-transplantation. Grafted cells maintained transgene expression throughout the 4-week period. At 1 week there was widespread migration of GFP + cells within the host retina and at 2 weeks evidence of neuronal differentiation (as shown by both marker expression and cell morphology), although integration at 4 weeks was limited to syngeneic recipients. Because brain-derived neural progenitor cells exhibit both neuronal and astrocytic differentiation in diseased and normal host retina, these cells provide a useful tool for studies of retinal regeneration.
ISSN:0042-6989
1878-5646
DOI:10.1016/S0042-6989(03)00235-9