Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)

The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of helminthology 2010-06, Vol.84 (2), p.136-142
Main Authors: dos Santos, K.R., Carlos, B.C., Paduan, K.S., Kadri, S.M., Barrella, T.H., Amarante, M.R.V., Ribolla, P.E.M., da Silva, R.J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brazil
Cluster Analysis
Colubridae - parasitology
DNA, Helminth - chemistry
DNA, Helminth - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Female
Fundamental and applied biological sciences. Psychology
Invertebrates
Molecular Sequence Data
Morphology. Histology. Cytology
Nemathelminthia. Plathelmintha
Phylogeny
RNA, Ribosomal, 18S - genetics
Sequence Analysis, DNA
Strongyloides - anatomy & histology
Strongyloides - genetics
Strongyloides - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lençóis Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X09990381