Loading…
Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia
In regulation of the developmental process, the balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, and increased apoptosis of placental trophoblasts can cause a variety of gynecological diseases. Members of the immortalization‐up...
Saved in:
| Published in: | Journal of cellular biochemistry 2010-05, Vol.110 (2), p.522-530 |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93 |
| container_end_page | 530 |
| container_issue | 2 |
| container_start_page | 522 |
| container_title | Journal of cellular biochemistry |
| container_volume | 110 |
| creator | Jeon, Su Yeon Lee, Hyun-Jung Park, Ji Myeong Jung, Hyun Min Yoo, Jung Ki Lee, Hey-Jin Lee, Jong-Sung CHA, Dong-Hyun Kim, Jin Kyeoung Kim, Gi Jin |
| description | In regulation of the developmental process, the balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, and increased apoptosis of placental trophoblasts can cause a variety of gynecological diseases. Members of the immortalization‐upregulated protein (IMUP) family are nuclear proteins implicated in SV40‐mediated immortalization and cellular proliferation; however, the mechanisms by which their expression is regulated in placental development are still unknown. We compared IMUP‐2 expression in normal and pre‐eclamptic placental tissues and evaluated the function of IMUP‐2 in HTR‐8/SVneo trophoblast cells under hypoxic conditions. IMUP‐2 was expressed in syncytiotrophoblasts and syncytial knots of the placental villi. IMUP‐2 expression was significantly higher in preterm pre‐eclampsia patients than in patients who went to term (P |
| doi_str_mv | 10.1002/jcb.22568 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733933257</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733933257</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93</originalsourceid><addsrcrecordid>eNp1kE1PFTEUQBujkSe68A-Y7oRFoV_ztdQXhEeeSAjExE3Tdm59xZnp2HYij1_v4AN2ru7innuSexB6z-gRo5Qf31pzxHlR1i_QgtGmIrKU8iVa0EpQwgXje-hNSreU0qYR_DXa41QKzmW5QPerwUbQCVrs-z7ErDt_r7MPA5nGCD-nTud5N8aQwQ-Y44PV15tLwg-x2eLNdgx3XmM_tJOFhPUYxhySTzg4nDeAcwzjJphOp4z18KABArbT_Zi8foteOd0lePc499HNl5Pr5RlZfztdLT-tiRUlr4k1VBrKWC2MZo4WbWWFAWMY4yUv60ZWjgKrXQPCFY2xXGrdzJArXGt424h99HHnnZ_4PUHKqvfJQtfpAcKUVCVEIwQvqpk83JE2hpQiODVG3-u4VYyqh9JqLq3-lZ7ZD4_WyfTQPpNPaWfgeAf88R1s_29S58vPT0qyu_Apw93zhY6_VFmJqlDfL06VXJ-fVVcXP9S1-AtCt5jG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733933257</pqid></control><display><type>article</type><title>Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia</title><source>Wiley</source><creator>Jeon, Su Yeon ; Lee, Hyun-Jung ; Park, Ji Myeong ; Jung, Hyun Min ; Yoo, Jung Ki ; Lee, Hey-Jin ; Lee, Jong-Sung ; CHA, Dong-Hyun ; Kim, Jin Kyeoung ; Kim, Gi Jin</creator><creatorcontrib>Jeon, Su Yeon ; Lee, Hyun-Jung ; Park, Ji Myeong ; Jung, Hyun Min ; Yoo, Jung Ki ; Lee, Hey-Jin ; Lee, Jong-Sung ; CHA, Dong-Hyun ; Kim, Jin Kyeoung ; Kim, Gi Jin</creatorcontrib><description>In regulation of the developmental process, the balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, and increased apoptosis of placental trophoblasts can cause a variety of gynecological diseases. Members of the immortalization‐upregulated protein (IMUP) family are nuclear proteins implicated in SV40‐mediated immortalization and cellular proliferation; however, the mechanisms by which their expression is regulated in placental development are still unknown. We compared IMUP‐2 expression in normal and pre‐eclamptic placental tissues and evaluated the function of IMUP‐2 in HTR‐8/SVneo trophoblast cells under hypoxic conditions. IMUP‐2 was expressed in syncytiotrophoblasts and syncytial knots of the placental villi. IMUP‐2 expression was significantly higher in preterm pre‐eclampsia patients than in patients who went to term (P < 0.001); however, we observed no differences in IMUP‐2 expression between normal term patients with and without pre‐eclampsia. Hypoxic conditions increased apoptosis of HTR8/SVneo trophoblast cells and induced IMUP‐2 expression. Also, apoptosis of HTR‐8/SVneo trophoblast cells was increased after IMUP‐2 gene transfection. These results suggest that IMUP‐2 expression is specifically elevated in preterm pre‐eclampsia and under hypoxic conditions, and that IMUP‐2 induces apoptosis of the trophoblast. Therefore, IMUP‐2 might have functional involvement in placental development and gynecological diseases such as pre‐eclampsia. J. Cell. Biochem. 110: 522–530, 2010. © 2010 Wiley‐Liss, Inc.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.22568</identifier><identifier>PMID: 20432246</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>apoptosis ; Apoptosis - physiology ; Base Sequence ; Blotting, Western ; Case-Control Studies ; Cell Line, Transformed ; DNA Primers ; Female ; Humans ; hypoxia ; Hypoxia - metabolism ; Hypoxia - pathology ; immortalization-upregulated proteins ; In Situ Hybridization ; Nuclear Proteins - physiology ; pre-eclampsia ; Pre-Eclampsia - metabolism ; Pre-Eclampsia - pathology ; Pregnancy ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription Factors - physiology ; trophoblast ; Trophoblasts - metabolism ; Trophoblasts - pathology</subject><ispartof>Journal of cellular biochemistry, 2010-05, Vol.110 (2), p.522-530</ispartof><rights>Copyright © 2010 Wiley‐Liss, Inc.</rights><rights>(c) 2010 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93</citedby><cites>FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20432246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeon, Su Yeon</creatorcontrib><creatorcontrib>Lee, Hyun-Jung</creatorcontrib><creatorcontrib>Park, Ji Myeong</creatorcontrib><creatorcontrib>Jung, Hyun Min</creatorcontrib><creatorcontrib>Yoo, Jung Ki</creatorcontrib><creatorcontrib>Lee, Hey-Jin</creatorcontrib><creatorcontrib>Lee, Jong-Sung</creatorcontrib><creatorcontrib>CHA, Dong-Hyun</creatorcontrib><creatorcontrib>Kim, Jin Kyeoung</creatorcontrib><creatorcontrib>Kim, Gi Jin</creatorcontrib><title>Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>In regulation of the developmental process, the balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, and increased apoptosis of placental trophoblasts can cause a variety of gynecological diseases. Members of the immortalization‐upregulated protein (IMUP) family are nuclear proteins implicated in SV40‐mediated immortalization and cellular proliferation; however, the mechanisms by which their expression is regulated in placental development are still unknown. We compared IMUP‐2 expression in normal and pre‐eclamptic placental tissues and evaluated the function of IMUP‐2 in HTR‐8/SVneo trophoblast cells under hypoxic conditions. IMUP‐2 was expressed in syncytiotrophoblasts and syncytial knots of the placental villi. IMUP‐2 expression was significantly higher in preterm pre‐eclampsia patients than in patients who went to term (P < 0.001); however, we observed no differences in IMUP‐2 expression between normal term patients with and without pre‐eclampsia. Hypoxic conditions increased apoptosis of HTR8/SVneo trophoblast cells and induced IMUP‐2 expression. Also, apoptosis of HTR‐8/SVneo trophoblast cells was increased after IMUP‐2 gene transfection. These results suggest that IMUP‐2 expression is specifically elevated in preterm pre‐eclampsia and under hypoxic conditions, and that IMUP‐2 induces apoptosis of the trophoblast. Therefore, IMUP‐2 might have functional involvement in placental development and gynecological diseases such as pre‐eclampsia. J. Cell. Biochem. 110: 522–530, 2010. © 2010 Wiley‐Liss, Inc.</description><subject>apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Base Sequence</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>Cell Line, Transformed</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Humans</subject><subject>hypoxia</subject><subject>Hypoxia - metabolism</subject><subject>Hypoxia - pathology</subject><subject>immortalization-upregulated proteins</subject><subject>In Situ Hybridization</subject><subject>Nuclear Proteins - physiology</subject><subject>pre-eclampsia</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pre-Eclampsia - pathology</subject><subject>Pregnancy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transcription Factors - physiology</subject><subject>trophoblast</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - pathology</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp1kE1PFTEUQBujkSe68A-Y7oRFoV_ztdQXhEeeSAjExE3Tdm59xZnp2HYij1_v4AN2ru7innuSexB6z-gRo5Qf31pzxHlR1i_QgtGmIrKU8iVa0EpQwgXje-hNSreU0qYR_DXa41QKzmW5QPerwUbQCVrs-z7ErDt_r7MPA5nGCD-nTud5N8aQwQ-Y44PV15tLwg-x2eLNdgx3XmM_tJOFhPUYxhySTzg4nDeAcwzjJphOp4z18KABArbT_Zi8foteOd0lePc499HNl5Pr5RlZfztdLT-tiRUlr4k1VBrKWC2MZo4WbWWFAWMY4yUv60ZWjgKrXQPCFY2xXGrdzJArXGt424h99HHnnZ_4PUHKqvfJQtfpAcKUVCVEIwQvqpk83JE2hpQiODVG3-u4VYyqh9JqLq3-lZ7ZD4_WyfTQPpNPaWfgeAf88R1s_29S58vPT0qyu_Apw93zhY6_VFmJqlDfL06VXJ-fVVcXP9S1-AtCt5jG</recordid><startdate>20100515</startdate><enddate>20100515</enddate><creator>Jeon, Su Yeon</creator><creator>Lee, Hyun-Jung</creator><creator>Park, Ji Myeong</creator><creator>Jung, Hyun Min</creator><creator>Yoo, Jung Ki</creator><creator>Lee, Hey-Jin</creator><creator>Lee, Jong-Sung</creator><creator>CHA, Dong-Hyun</creator><creator>Kim, Jin Kyeoung</creator><creator>Kim, Gi Jin</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100515</creationdate><title>Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia</title><author>Jeon, Su Yeon ; Lee, Hyun-Jung ; Park, Ji Myeong ; Jung, Hyun Min ; Yoo, Jung Ki ; Lee, Hey-Jin ; Lee, Jong-Sung ; CHA, Dong-Hyun ; Kim, Jin Kyeoung ; Kim, Gi Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Base Sequence</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>Cell Line, Transformed</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Humans</topic><topic>hypoxia</topic><topic>Hypoxia - metabolism</topic><topic>Hypoxia - pathology</topic><topic>immortalization-upregulated proteins</topic><topic>In Situ Hybridization</topic><topic>Nuclear Proteins - physiology</topic><topic>pre-eclampsia</topic><topic>Pre-Eclampsia - metabolism</topic><topic>Pre-Eclampsia - pathology</topic><topic>Pregnancy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transcription Factors - physiology</topic><topic>trophoblast</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeon, Su Yeon</creatorcontrib><creatorcontrib>Lee, Hyun-Jung</creatorcontrib><creatorcontrib>Park, Ji Myeong</creatorcontrib><creatorcontrib>Jung, Hyun Min</creatorcontrib><creatorcontrib>Yoo, Jung Ki</creatorcontrib><creatorcontrib>Lee, Hey-Jin</creatorcontrib><creatorcontrib>Lee, Jong-Sung</creatorcontrib><creatorcontrib>CHA, Dong-Hyun</creatorcontrib><creatorcontrib>Kim, Jin Kyeoung</creatorcontrib><creatorcontrib>Kim, Gi Jin</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeon, Su Yeon</au><au>Lee, Hyun-Jung</au><au>Park, Ji Myeong</au><au>Jung, Hyun Min</au><au>Yoo, Jung Ki</au><au>Lee, Hey-Jin</au><au>Lee, Jong-Sung</au><au>CHA, Dong-Hyun</au><au>Kim, Jin Kyeoung</au><au>Kim, Gi Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J. Cell. Biochem</addtitle><date>2010-05-15</date><risdate>2010</risdate><volume>110</volume><issue>2</issue><spage>522</spage><epage>530</epage><pages>522-530</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>In regulation of the developmental process, the balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, and increased apoptosis of placental trophoblasts can cause a variety of gynecological diseases. Members of the immortalization‐upregulated protein (IMUP) family are nuclear proteins implicated in SV40‐mediated immortalization and cellular proliferation; however, the mechanisms by which their expression is regulated in placental development are still unknown. We compared IMUP‐2 expression in normal and pre‐eclamptic placental tissues and evaluated the function of IMUP‐2 in HTR‐8/SVneo trophoblast cells under hypoxic conditions. IMUP‐2 was expressed in syncytiotrophoblasts and syncytial knots of the placental villi. IMUP‐2 expression was significantly higher in preterm pre‐eclampsia patients than in patients who went to term (P < 0.001); however, we observed no differences in IMUP‐2 expression between normal term patients with and without pre‐eclampsia. Hypoxic conditions increased apoptosis of HTR8/SVneo trophoblast cells and induced IMUP‐2 expression. Also, apoptosis of HTR‐8/SVneo trophoblast cells was increased after IMUP‐2 gene transfection. These results suggest that IMUP‐2 expression is specifically elevated in preterm pre‐eclampsia and under hypoxic conditions, and that IMUP‐2 induces apoptosis of the trophoblast. Therefore, IMUP‐2 might have functional involvement in placental development and gynecological diseases such as pre‐eclampsia. J. Cell. Biochem. 110: 522–530, 2010. © 2010 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20432246</pmid><doi>10.1002/jcb.22568</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-2312 |
| ispartof | Journal of cellular biochemistry, 2010-05, Vol.110 (2), p.522-530 |
| issn | 0730-2312 1097-4644 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733933257 |
| source | Wiley |
| subjects | apoptosis Apoptosis - physiology Base Sequence Blotting, Western Case-Control Studies Cell Line, Transformed DNA Primers Female Humans hypoxia Hypoxia - metabolism Hypoxia - pathology immortalization-upregulated proteins In Situ Hybridization Nuclear Proteins - physiology pre-eclampsia Pre-Eclampsia - metabolism Pre-Eclampsia - pathology Pregnancy Reverse Transcriptase Polymerase Chain Reaction Transcription Factors - physiology trophoblast Trophoblasts - metabolism Trophoblasts - pathology |
| title | Increased immortalization-upregulated protein 2 (IMUP-2) by hypoxia induces apoptosis of the trophoblast and pre-eclampsia |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T12%3A59%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20immortalization-upregulated%20protein%202%20(IMUP-2)%20by%20hypoxia%20induces%20apoptosis%20of%20the%20trophoblast%20and%20pre-eclampsia&rft.jtitle=Journal%20of%20cellular%20biochemistry&rft.au=Jeon,%20Su%20Yeon&rft.date=2010-05-15&rft.volume=110&rft.issue=2&rft.spage=522&rft.epage=530&rft.pages=522-530&rft.issn=0730-2312&rft.eissn=1097-4644&rft_id=info:doi/10.1002/jcb.22568&rft_dat=%3Cproquest_cross%3E733933257%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3628-cb04b01183ba1f05d7c3bebb1126268947f0e18f9e3f59bc24aa9d7cf5fdb2d93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733933257&rft_id=info:pmid/20432246&rfr_iscdi=true |