Separation of Peptides and Oligonucleotides Using a Monolithic Polymer Layer and Pressurized Planar Electrophoresis and Electrochromatography

The rapid separation of mixtures of six peptides using porous polymer monolithic layers in electrophoresis and pressurized planar electrochromatography modes has been achieved. The separations in the former mode were performed on a generic hydrophobic poly(butyl methacrylate-co-ethylene dimethacryla...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2010-05, Vol.82 (9), p.3445-3448
Main Authors: Woodward, Scott D, Urbanova, Iva, Nurok, David, Svec, Frantisek
Format: Article
Language:English
Subjects:
Air Pressure
Analytical chemistry
Chemical compounds
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Chromatography, Micellar Electrokinetic Capillary
Electrophoresis
Exact sciences and technology
Oligonucleotides - chemistry
Other chromatographic methods
Peptides
Peptides - chemistry
Polymers
Polymers - chemistry
Porosity
Proteomics
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Summary:The rapid separation of mixtures of six peptides using porous polymer monolithic layers in electrophoresis and pressurized planar electrochromatography modes has been achieved. The separations in the former mode were performed on a generic hydrophobic poly(butyl methacrylate-co-ethylene dimethacrylate) layer with no ionizable functionalities and required 2 min. This layer also enabled the separation of three oligonucleotides. The separation in the pressurized planar electrochromatographic mode was carried out using a negatively charged layer prepared via cografting of 2-acrylamido-2-methyl-1-propanesulfonic acid and 2-hydroxyethyl methacrylate on top of the generic hydrophobic monolith and was completed in 1 min.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac100392b