Is the Cell Death in Mesial Temporal Sclerosis Apoptotic?

• Published in: Epilepsia (Copenhagen) 2003-06, Vol.44 (6), p.778-784
• Main Authors: Uysal, Hilmi, Cevik, Isin Unal, Soylemezoglu, Figen, Elibol, Bulent, Ozdemir, Yasemin Gursoy, Evrenkaya, Tulay, Saygi, Serap, Dalkara, Turgay
• Format: Article
• Language: English
• Subjects: Actin Cytoskeleton - ultrastructure; Adult; Age of Onset; Apoptosis; Astrocytes - cytology; Astrocytes - pathology; Bax; Bcl‐2; Biological and medical sciences; Caspases; Cell Count; Cell Death; DNA Fragmentation; Epilepsy; Epilepsy, Temporal Lobe - diagnosis; Epilepsy, Temporal Lobe - metabolism; Epilepsy, Temporal Lobe - pathology; Female; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy; Hippocampus - cytology; Hippocampus - pathology; Humans; Immunohistochemistry; In Situ Nick-End Labeling - methods; Male; Medical sciences; Nervous system (semeiology, syndromes); Neurology; Neurons - cytology; Neurons - pathology; Sclerosis; Temporal Lobe - pathology
• ISSN: 0013-9580; 1528-1167
• DOI: 10.1046/j.1528-1157.2003.37402.x

Purpose: Mesial temporal sclerosis (MTS) is characterized by neuronal loss in the hippocampus. Studies on experimental models and patients with intractable epilepsy suggest that apoptosis may be involved in neuronal death induced by recurrent seizures. Methods: We searched evidence for apoptotic cell death in temporal lobes resected from drug‐resistant epilepsy patients with MTS by using the terminal deoxynucleotidyl transferase (TdT) and digoxigenin‐11‐dUTP (TUNEL) method and immunohistochemistry for Bcl‐2, Bax, and caspase‐cleaved actin fragment, fractin. The temporal lobe specimens were obtained from 15 patients (six women and nine men; mean age, 29 ± 8 years). Results: Unlike that in normal adult brain, we observed Bcl‐2 immunoreactivity in some of the remaining neurons dispersed throughout the hippocampus proper as well as in most of the reactive astroglia. Bax immunopositivity was increased in almost all neurons. Fractin immunostaining, an indicator of caspase activity, was detected in ∼10% of these neurons. Des pite increased Bax expression and activation of caspases, we could not find evidence for DNA fragmentation by TUNEL staining. We also could not detect typical apoptotic changes in nuclear morphology by Hoechst‐33258 or hematoxylin counterstaining. Conclusions: These data suggest that either apoptosis is not involved in cell loss in MTS, or a very slow rate of cell demise may have precluded detecting TUNEL‐positive neurons dying through apoptosis. Increased Bax expression and activation of caspases support the latter possibility.