Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2010-03, Vol.32 (3), p.367-371
Main Authors: Wei, Zheng-Li, Ogawa, Ryohei, Takasaki, Ichiro, Zhao, Qing-Li, Zheng, Hua-Chuan, Ahmed, Kanwal, Hassan, Mariame A, Kondo, Takashi
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cell culture
Combined treatment
Dextrans - metabolism
Electroporation - methods
Fever
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluorescein-5-isothiocyanate - metabolism
Fundamental and applied biological sciences. Psychology
HCT116 Cells
Heat-Shock Response
HeLa Cells
Humans
Intracellular Space - metabolism
Life Sciences
Luciferases - genetics
Luciferases - metabolism
Microbiology
Original Research Paper
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcription, Genetic
Transfection - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-009-0164-5