Use of different buffers for detection and separation in determination of physio-active components in oolong tea infusion by CZE with amperometric detection

With a view of simultaneous determination of physio-active ingredients in oolong tea infusion: sugars, amino acids, epigallocatechin gallate and ascorbic acid, a novel CZE with amperometric detection method was studied. Operated in a wall-jet configuration, 100 mmol/L NaOH was used in detecting cell...

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Bibliographic Details
Published in:Journal of separation science 2010-05, Vol.33 (9), p.1312-1318
Main Authors: Yang, Zhiyong, Li, Zicheng, Zhu, Jinkun, Wang, Qingjiang, He, Pingang, Fang, Yuzhi
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Amperometric detection
Ascorbic Acid - analysis
Biological and medical sciences
Buffers
Carbohydrates - analysis
Catechin - analogs & derivatives
Catechin - analysis
Coffee, tea and other stimulative beverage industries
Electrical measurements
Electrochemistry - methods
Electrodes
Electrophoresis, Capillary - methods
Food industries
Fundamental and applied biological sciences. Psychology
General pharmacology
Infusion
Limit of Detection
Linearity
Medical sciences
Oolong tea infusion
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Physio-active components
Reproducibility of Results
Separation
Systems stability
Tea
Tea - chemistry
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Summary:With a view of simultaneous determination of physio-active ingredients in oolong tea infusion: sugars, amino acids, epigallocatechin gallate and ascorbic acid, a novel CZE with amperometric detection method was studied. Operated in a wall-jet configuration, 100 mmol/L NaOH was used in detecting cell to lead the electrocatalysis oxidation behaviors of the analytes on a 300 μm diameter copper-disc electrode (working electrode), while in separating capillary, a mild alkaline running buffer consisting in a mixture of 30 mmol/L borate and 40 mmol/L phosphates charged and carried analytes to detecting end. The methodology research was performed for system stability and suitability. Under the optimal CE conditions, analytes could be separated within moderate time period. Good linearity between peak area and concentration existed over three orders of magnitude; lower RSD and LOD were achieved. The oolong tea infusion was assayed and result was satisfactory.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900586