Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid

2,5-Furandicarboxylic acid (FDCA) is a promising bio-based platform chemical that may serve as a ‘green’ substitute for terephthalate in polyesters. Recently, a novel HMF/furfural oxidoreductase from Cupriavidus basilensis HMF14 was identified that converts 5-(hydroxymethyl)furfural (HMF) into FDCA....

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Bibliographic Details
Published in:Bioresource technology 2010-08, Vol.101 (16), p.6291-6296
Main Authors: Koopman, Frank, Wierckx, Nick, de Winde, Johannes H., Ruijssenaars, Harald J.
Format: Article
Language:English
Subjects:
2,5-Furandicarboxylic acid
Biocatalysis
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Biotransformation
Dicarboxylic Acids - metabolism
Fundamental and applied biological sciences. Psychology
Furaldehyde - analogs & derivatives
Furaldehyde - metabolism
Furans - metabolism
HMF/furfural oxidoreductase
Methods. Procedures. Technologies
Pseudomonas putida - metabolism
Pseudomonas putida S12
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Summary:2,5-Furandicarboxylic acid (FDCA) is a promising bio-based platform chemical that may serve as a ‘green’ substitute for terephthalate in polyesters. Recently, a novel HMF/furfural oxidoreductase from Cupriavidus basilensis HMF14 was identified that converts 5-(hydroxymethyl)furfural (HMF) into FDCA. The hmfH gene encoding this oxidoreductase was introduced into Pseudomonas putida S12 and the resulting whole-cell biocatalyst was employed to produce FDCA from HMF. In fed-batch experiments using glycerol as the carbon source, 30.1 g l −1 of FDCA was produced from HMF at a yield of 97%. FDCA was recovered from the culture broth as a 99.4% pure dry powder, at 76% recovery using acid precipitation and subsequent tetrahydrofuran extraction.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.03.050