Treatment of intracerebral haemorrhage in rats with intraventricular transplantation of human amniotic epithelial cells

We explored the effects on brain oedema and neurological functional recovery after transplantation of hAECs (human amniotic epithelial cells) into the lateral ventricle of rats with ICH (intracerebral haemorrhage). hAECs were isolated from human term placenta and seeded for primary culture. We deliv...

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Bibliographic Details
Published in:Cell biology international 2010-06, Vol.34 (6), p.573-577
Main Authors: Dong, Wanli, Chen, Haiyun, Yang, Xinxin, Guo, Lihe, Hui, Guozhen
Format: Article
Language:English
Subjects:
Amnion - cytology
Animals
Behavior, Animal
Brain Edema - pathology
CD11b Antigen - metabolism
Cells, Cultured
cerebral haemorrhage
Cerebral Hemorrhage - therapy
Disease Models, Animal
epithelial cell
Epithelial Cells - transplantation
Female
Fluorescent Dyes - chemistry
Humans
Injections, Intraventricular
Intermediate Filament Proteins - metabolism
Microglia - cytology
Microglia - metabolism
Motor Activity - physiology
Nerve Tissue Proteins - metabolism
Nestin
rat transplantation
Rats
Rats, Sprague-Dawley
Vimentin - metabolism
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Summary:We explored the effects on brain oedema and neurological functional recovery after transplantation of hAECs (human amniotic epithelial cells) into the lateral ventricle of rats with ICH (intracerebral haemorrhage). hAECs were isolated from human term placenta and seeded for primary culture. We delivered hAECs labelled with Hoechst33258 and transfected with EGFP (enhanced green fluorescent protein) gene using lentiviral vectors into ICH rat models. The behaviour of the animals and brain oedema were evaluated after 28 days, and brain sections were made for morphological and immunohistochemical analyses with fluorescence microscopy. Our results were as follows. Transplanted hAECs were observed along the lateral wall and survived for at least 4 weeks. Some of the cells were stained with human specific antibody to vimentin and nestin. Around the injury site, activated microglia stained with OX42 were reduced. The water content of ICH rats decreased in the treatment group. The behaviour test scores were improved in the treatment group compared with those in the control groups. In conclusion, hAECs cannot only survive in the lateral ventricle of ICH rats after transplantation, but also express vimentin and nestin. hAEC transplantation reduced brain oedema and improved the motor deficits of ICH rats.
ISSN:1065-6995
1095-8355
DOI:10.1042/CBI20090248