Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy

In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans ( C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a pow...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2010-07, Vol.41 (5), p.444-447
Main Authors: Tserevelakis, G.J., Filippidis, G., Krmpot, A.J., Vlachos, M., Fotakis, C., Tavernarakis, N.
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans
Caenorhabditis elegans - cytology
Caenorhabditis elegans - growth & development
Embryo, Nonmammalian - cytology
Embryogenesis
Embryonic Development
Image Processing, Computer-Assisted - methods
Imaging
Lasers
Microscopy - methods
THG
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Summary:In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans ( C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex developmental process of C. elegans embryogenesis.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2010.02.006