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Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome

Objective The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. Methods TLR7, 8, and...

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Published in:Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2010-06, Vol.109 (6), p.844-850
Main Authors: Zheng, Lingyan, MD, Zhang, Zhiyuan, MD, PhD, Yu, Chuangqi, MD, PhD, Yang, Chi, MD, PhD
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container_title Oral surgery, oral medicine, oral pathology, oral radiology and endodontics
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creator Zheng, Lingyan, MD
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description Objective The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. Methods TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. Results TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively ( P < .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands ( P < .05). Conclusion TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.
doi_str_mv 10.1016/j.tripleo.2010.01.006
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Methods TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. Results TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively ( P &lt; .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands ( P &lt; .05). Conclusion TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.</description><identifier>ISSN: 1079-2104</identifier><identifier>EISSN: 1528-395X</identifier><identifier>DOI: 10.1016/j.tripleo.2010.01.006</identifier><identifier>PMID: 20399690</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Case-Control Studies ; Dentistry ; Female ; Humans ; Immunohistochemistry ; Leukocytes, Mononuclear - metabolism ; Male ; Medical sciences ; Middle Aged ; Otorhinolaryngology. Stomatology ; Parotid Gland - metabolism ; Reference Values ; RNA, Messenger - analysis ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Sjogren's Syndrome - genetics ; Sjogren's Syndrome - metabolism ; Statistics, Nonparametric ; Surgery ; Toll-Like Receptor 7 - genetics ; Toll-Like Receptor 7 - metabolism ; Toll-Like Receptor 8 - genetics ; Toll-Like Receptor 8 - metabolism ; Toll-Like Receptor 9 - genetics ; Toll-Like Receptor 9 - metabolism ; Toll-Like Receptors - genetics ; Toll-Like Receptors - metabolism</subject><ispartof>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 2010-06, Vol.109 (6), p.844-850</ispartof><rights>Mosby, Inc.</rights><rights>2010 Mosby, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Mosby, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-9eb6af573d690f4960f6513c4c30d6c83b3af7e87c8851d3576e120990e935153</citedby><cites>FETCH-LOGICAL-c445t-9eb6af573d690f4960f6513c4c30d6c83b3af7e87c8851d3576e120990e935153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=22853812$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20399690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Lingyan, MD</creatorcontrib><creatorcontrib>Zhang, Zhiyuan, MD, PhD</creatorcontrib><creatorcontrib>Yu, Chuangqi, MD, PhD</creatorcontrib><creatorcontrib>Yang, Chi, MD, PhD</creatorcontrib><title>Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome</title><title>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</title><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><description>Objective The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. Methods TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. Results TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively ( P &lt; .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands ( P &lt; .05). Conclusion TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Dentistry</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Parotid Gland - metabolism</subject><subject>Reference Values</subject><subject>RNA, Messenger - analysis</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>Sjogren's Syndrome - genetics</subject><subject>Sjogren's Syndrome - metabolism</subject><subject>Statistics, Nonparametric</subject><subject>Surgery</subject><subject>Toll-Like Receptor 7 - genetics</subject><subject>Toll-Like Receptor 7 - metabolism</subject><subject>Toll-Like Receptor 8 - genetics</subject><subject>Toll-Like Receptor 8 - metabolism</subject><subject>Toll-Like Receptor 9 - genetics</subject><subject>Toll-Like Receptor 9 - metabolism</subject><subject>Toll-Like Receptors - genetics</subject><subject>Toll-Like Receptors - metabolism</subject><issn>1079-2104</issn><issn>1528-395X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAQhi0EomXhEUC-oF6aZRzHTnyhQlWBSiv10CJxs7zOBDnN2sHOIvbFeAFeDEcbqMSlp7Gsb2Z-fUPIawZrBky-69dTdOOAYV1C_gO2BpBPyCkTZVNwJb4-zW-oVVEyqE7Ii5R6yARX6jk5KSFXqeCUbK5-jhFTcsHT0NG7MAzF4O6RRrQ4TiEmWp_T5pwa31JFnadjdDsTD_S2__3rW0R_lmg6-DaGHb4kzzozJHy11BX58vHq7vJzsbn5dH35YVPYqhJToXArTSdq3uYIXaUkdFIwbivLoZW24Vtuuhqb2jaNYC0XtURWglKAigsm-IqcHeeOMXzfY5r0ziWLw2A8hn3SNc8ClKzKTIojaWNIKWKnl_iagZ496l4vHvXsUQPTs6UVebNs2G932P7r-isuA28XwCRrhi4ab1164MpG8IbNAS6OHGYfPxxGnaxDb7F12fCk2-AejfL-vwl2cN7lpfd4wNSHffRZtmY6lRr07Xz0-eYsnxtKCfwPb3WmvQ</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Zheng, Lingyan, MD</creator><creator>Zhang, Zhiyuan, MD, PhD</creator><creator>Yu, Chuangqi, MD, PhD</creator><creator>Yang, Chi, MD, PhD</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100601</creationdate><title>Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome</title><author>Zheng, Lingyan, MD ; Zhang, Zhiyuan, MD, PhD ; Yu, Chuangqi, MD, PhD ; Yang, Chi, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-9eb6af573d690f4960f6513c4c30d6c83b3af7e87c8851d3576e120990e935153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Dentistry</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Parotid Gland - metabolism</topic><topic>Reference Values</topic><topic>RNA, Messenger - analysis</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>Sjogren's Syndrome - genetics</topic><topic>Sjogren's Syndrome - metabolism</topic><topic>Statistics, Nonparametric</topic><topic>Surgery</topic><topic>Toll-Like Receptor 7 - genetics</topic><topic>Toll-Like Receptor 7 - metabolism</topic><topic>Toll-Like Receptor 8 - genetics</topic><topic>Toll-Like Receptor 8 - metabolism</topic><topic>Toll-Like Receptor 9 - genetics</topic><topic>Toll-Like Receptor 9 - metabolism</topic><topic>Toll-Like Receptors - genetics</topic><topic>Toll-Like Receptors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Lingyan, MD</creatorcontrib><creatorcontrib>Zhang, Zhiyuan, MD, PhD</creatorcontrib><creatorcontrib>Yu, Chuangqi, MD, PhD</creatorcontrib><creatorcontrib>Yang, Chi, MD, PhD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Lingyan, MD</au><au>Zhang, Zhiyuan, MD, PhD</au><au>Yu, Chuangqi, MD, PhD</au><au>Yang, Chi, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome</atitle><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>109</volume><issue>6</issue><spage>844</spage><epage>850</epage><pages>844-850</pages><issn>1079-2104</issn><eissn>1528-395X</eissn><abstract>Objective The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. Methods TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. Results TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively ( P &lt; .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands ( P &lt; .05). Conclusion TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>20399690</pmid><doi>10.1016/j.tripleo.2010.01.006</doi><tpages>7</tpages></addata></record>
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identifier ISSN: 1079-2104
ispartof Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 2010-06, Vol.109 (6), p.844-850
issn 1079-2104
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source ScienceDirect Freedom Collection 2022-2024
subjects Adult
Aged
Biological and medical sciences
Case-Control Studies
Dentistry
Female
Humans
Immunohistochemistry
Leukocytes, Mononuclear - metabolism
Male
Medical sciences
Middle Aged
Otorhinolaryngology. Stomatology
Parotid Gland - metabolism
Reference Values
RNA, Messenger - analysis
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Sjogren's Syndrome - genetics
Sjogren's Syndrome - metabolism
Statistics, Nonparametric
Surgery
Toll-Like Receptor 7 - genetics
Toll-Like Receptor 7 - metabolism
Toll-Like Receptor 8 - genetics
Toll-Like Receptor 8 - metabolism
Toll-Like Receptor 9 - genetics
Toll-Like Receptor 9 - metabolism
Toll-Like Receptors - genetics
Toll-Like Receptors - metabolism
title Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome
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