Metabolite analysis in Curcuma domestica using various GC-MS and LC-MS separation and detection techniques

The metabolic profile of polar (methanol) and non‐polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC‐FID, GC‐MS, HR‐GC‐MS and analytical HPLC‐ESI‐MS/MS by means of LTQ‐Orbitrap technology. The major...

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Bibliographic Details
Published in:Biomedical chromatography 2009-09, Vol.23 (9), p.951-965
Main Authors: Herebian, Diran, Choi, Jeong-Heui, Abd El-Aty, A. M., Shim, Jae-Han, Spiteller, Michael
Format: Article
Language:English
Subjects:
(HR)-GC-MS
Chromatography, High Pressure Liquid - methods
Curcuma - chemistry
Curcuma - metabolism
Curcuma domestica
Curcumin - analogs & derivatives
Curcumin - analysis
Curcumin - isolation & purification
Diarylheptanoids
Gas Chromatography-Mass Spectrometry - methods
Hexanes - chemistry
HPLC-ESI-MS/MS
LTQ-Orbitrap
metabolite profile
Methanol - chemistry
Plant Extracts - analysis
Plant Extracts - chemistry
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:The metabolic profile of polar (methanol) and non‐polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC‐FID, GC‐MS, HR‐GC‐MS and analytical HPLC‐ESI‐MS/MS by means of LTQ‐Orbitrap technology. The major non‐volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS‐based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1207