Adipose Tissue of Control and Ex-Obese Patients Exhibit Differences in Blood Vessel Content and Resident Mesenchymal Stem Cell Population

• Published in: Obesity surgery 2009-09, Vol.19 (9), p.1304-1312
• Main Authors: Baptista, Leandra Santos, da Silva, Karina Ribeiro, da Pedrosa, Carolina Silva Gouveia, Claudio-da-Silva, Cesar, Carneiro, João Regis Ivar, Aniceto, Marcelo, de Mello-Coelho, Valéria, Takiya, Christina Maeda, Rossi, Maria Isabel Doria, Borojevic, Radovan
• Format: Article
• Language: English
• Subjects: Adult; Blood vessels; Body fat; Body Mass Index; Case-Control Studies; Cell Count; Female; Gastrointestinal surgery; Humans; Medicine; Medicine & Public Health; Mesenchymal Stromal Cells - physiology; Middle Aged; Obesity; Obesity - pathology; Obesity - physiopathology; Obesity - surgery; PPAR gamma - metabolism; Stem cells; Subcutaneous Fat - blood supply; Subcutaneous Fat - metabolism; Subcutaneous Fat - pathology; Surgery; Weight Loss
• ISSN: 0960-8923; 1708-0428
• DOI: 10.1007/s11695-009-9899-2

Background The normal function of white adipose tissue is disturbed in obesity. After weight loss that follows bariatric surgery, ex-obese patients undergo plastic surgery to remove residual tissues and it is not known whether their adipose tissue returns to its original state. The aim of this study was to compare the white adipose tissue composition of ex-obese with control patients with regard to blood vessels and resident mesenchymal stem cells (MSC). Methods Quantification of blood vessels was performed on histological sections of adipose tissue stained with hematoxylin and eosin and for von Willebrand antigen. MSC were induced to the adipogenic and osteogenic lineages by specific inductive culture media. Expression of PPARγ2 was analyzed by reverse transcription polymerase chain reaction. Results Ex-obese adipose tissue showed a higher number ( p  = 0.0286) of small (107.3 ± 22.0) and large (22.5 ± 6.4) blood vessels, when compared to control patients (42.0 ± 24.4 and 7.2 ± 2.2, respectively) and they also occupied a larger area (control versus ex-obese, p  = 0.0286). Adipose tissue MSC from both groups of patients expressed PPARγ2 and were equally able to differentiate to the osteogenic lineage, but ex-obese MSC showed a higher adipogenic potential when induced in vitro ( p