Association Study and Mutation Analysis of Adiponectin Shows Association of Variants in APM1 with Complex Obesity in Women

Summary We performed an association study and mutation analysis of the adiponectin (APM1) gene to study its involvement in the development of obesity. We also studied the interaction with peroxisome proliferator‐activated receptor γ (PPARγ). 223 obese women and 87 healthy female control subjects wer...

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Bibliographic Details
Published in:Annals of human genetics 2009-09, Vol.73 (5), p.492-501
Main Authors: Beckers, Sigri, Peeters, Armand V., De Freitas, Fenna, Mertens, Ilse L., Verhulst, Stijn L., Haentjens, Dominique, Desager, Kristine N., Van Gaal, Luc F., Van Hul, Wim
Format: Article
Language:English
Subjects:
Adiponectin
Adiponectin - genetics
Adolescent
Adult
association study
Belgium
Case-Control Studies
Child
Cohort Studies
Female
Haplotypes
Humans
Mutation
mutation analysis
obesity
Obesity - genetics
Polymorphism, Single Nucleotide
PPAR gamma - genetics
SNP
women
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Summary:Summary We performed an association study and mutation analysis of the adiponectin (APM1) gene to study its involvement in the development of obesity. We also studied the interaction with peroxisome proliferator‐activated receptor γ (PPARγ). 223 obese women and 87 healthy female control subjects were used for association analysis. Mutation analysis was done on 95 morbidly obese adults and 123 overweight and obese children and adolescents. We selected 6 haplotype tagging SNPs in APM1 and the Pro12Ala variant (rs1805192) in PPARγ to study the interaction. The G allele of rs2241766 was more common in controls (cases 10.8% vs. controls 18.4%, nominal p = 0.011; OR = 0.57, nominal p = 0.018). The rs2241766/rs3774261 haplotype was also associated with obesity (nominal p = 0.004). Only the latter association remained significant after controlling for the False Discovery Rate. Resequencing of exon 2, exon 3 and intron 2 in 95 individuals did not reveal any SNPs in high linkage disequilibrium with rs2241766. No interaction with the Pro12Ala variant in PPARγ was detected. Mutation analysis of APM1 did not identify mutations. In conclusion, we found an association of an APM1 haplotype with obesity and found that APM1 mutations are not a common cause of monogenic obesity in our cohort.
ISSN:0003-4800
1469-1809
DOI:10.1111/j.1469-1809.2009.00532.x