Procedures in the 13C octanoic acid breath test for measurement of gastric emptying: analysis using Bland-Altman methods

• Published in: Scandinavian journal of gastroenterology 2010-08, Vol.45 (7-8), p.852-861
• Main Authors: Clegg, Miriam E., Shafat, Amir
• Format: Article
• Language: English
• Subjects: Adult; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Biological and medical sciences; Breath Tests - methods; C octanoic acid breath test; Caprylates; Female; gastric emptying; Gastric Emptying - physiology; Gastroenterology. Liver. Pancreas. Abdomen; half time; Humans; lag phase; Male; Medical sciences; Pharmacology. Drug treatments; Time Factors
• ISSN: 0036-5521; 1502-7708
• DOI: 10.3109/00365521.2010.483740

Abstract Objective. The 13C octanoic acid breath test (OBT) was first developed as an alternative method of measuring gastric emptying (GE) to scintigraphy. There has been much debate about the test duration and how often measurements need to be taken. This study aims to address these issues. Material and methods. For 78 GE tests using the 13C OBT, GE lag phase (Tlag) was calculated while sampling more frequently than the recommended every 15 min. Comparisons between Tlag were completed using Bland-Altman plots. Similarly, 4 or 6 h test durations were assessed to establish if they yield the same GE half time (Thalf). From one volunteer, samples were taken every 1 min for the first 30 min and then every 15 min until 6 h. GE times were then calculated using different combinations of sampling times. Evidence of a visible Tlag was also explored from this data. Results. Findings indicated that taking samples every 5 min for the first 30 min instead of every 15 min did not change the GE Tlag based on Bland-Altman plots. The correlation between these two methods was also high (r2 = 0.9957). The findings showed that the difference between the two sampling durations 4 and 6 h was large and the correlation between the methods was low (r2 = 0.8335). Samples taken at a rate of one breath per min indicated lack of a visible Tlag. Conclusions. Sampling for the 13C OBT should be completed every 15 min for 6 h.