Biofilms and chronic otitis media: an initial exploration into the role of biofilms in the pathogenesis of chronic otitis media

• Published in: American journal of otolaryngology 2010-07, Vol.31 (4), p.241-245
• Main Authors: Hoa, Michael, MD, Syamal, Mausumi, MS, Schaeffer, Michele A., BA, Sachdeva, Livjot, MS, Berk, Richard, PhD, Coticchia, James, MD
• Format: Article
• Language: English
• Subjects: Adenoidectomy; Adenoids - microbiology; Adenoids - pathology; Adenoids - surgery; Bacteria; Bacterial diseases; Biofilms; Biofilms - growth & development; Biological and medical sciences; Child; Child, Preschool; Chronic Disease; Cytokines; Ear diseases; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology; Ent and stomatologic bacterial diseases; Female; Human bacterial diseases; Humans; Infant; Infectious diseases; Male; Medical sciences; Microbiology; Microscopy, Electron, Scanning; Non tumoral diseases; Otitis Media - microbiology; Otitis Media - pathology; Otolaryngology; Otorhinolaryngology. Stomatology; Pathogenesis; Recurrence; Respiratory Mucosa - microbiology; Respiratory Mucosa - ultrastructure; Scanning electron microscopy; Streptococcus infections; Studies; Surgery
• ISSN: 0196-0709; 1532-818X
• DOI: 10.1016/j.amjoto.2009.02.015

Abstract Purpose The aim of the study was to compare the extent of biofilm infection in percentage of mucosal surface area of adenoids removed from children with otitis media with effusion (OME) vs those with recurrent acute otitis media (RAOM) and obstructive sleep apnea (OSA). Materials and methods Comparative microanatomical investigation of adenoid mucosa using scanning electron microscopy obtained from 30 children with OME, RAOM, and OSA was used in this study. Seventeen males and 13 females ranging in age from 9 months to 10 years were included in this study. Percentage of biofilm surface area involvement was the main measure. Results Adenoids removed from patients with OME had moderately dense mature biofilms covering the mucosal surface with a mean of 27.7% of their mucosal surface covered with mature biofilms. These results were distinct from results obtained from patients diagnosed with RAOM and OSA with means of 97.6% and 0.10% of their mucosal surfaces covered with mature biofilms, respectively. These differences were statistically significant at P < .0001. Conclusions Adenoids removed from patients with OME were characterized by distinctly different percentage of biofilm mucosal surface area coverage, with significantly more biofilm presence than OSA patients but significantly less biofilm presence than RAOM patients. Although previous investigations have supported a dominant role of nasopharyngeal biofilms in RAOM pathogenesis, these results suggest nasopharyngeal biofilms may play a different role in the pathogenesis of OME and that this clinical entity may be more multifactorial in nature.