Selective repression of c- fos gene transcription in rat embryo fibroblasts transformed by oncogenes E1A and cHa-ras

• Published in: Biochemical and biophysical research communications 2003-06, Vol.306 (2), p.483-487
• Main Authors: Abramova, M.V, Kukushkin, A.N, Svetlikova, S.B, Pospelova, T.V, Pospelov, V.A
• Format: Article
• Language: English
• Subjects: Adenovirus E1A Proteins - metabolism; Animals; Blotting, Western; c- fos Repression; Cell Line, Transformed; Cells, Cultured; Chromatin - metabolism; Chromatin remodeling; Dimerization; E1A and ras oncogenes; Fibroblasts - metabolism; Histone deacetylase; Histone Deacetylases - metabolism; Kinetics; Proto-Oncogene Proteins c-fos - metabolism; ras Proteins - metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Sodium butyrate; Sodium Oxybate - pharmacology; Transcription, Genetic
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(03)00996-3

Transformation of REF cells by oncogenes E1A and c Ha-ras leads to activation of AP-1 factor concomitantly with down-regulation of c- fos gene transcription. Here we addressed two issues: (i) how does transcription of Fos/Jun-regulated genes change in the cells lacking Fos-Jun heterodimers; (ii) to which extent HDAC-mediated chromatin reorganization does affect, apart from c- fos, transcription of some other early and late-response genes. To this end, we studied the kinetics of serum-stimulated transcription of c- fos, c- jun, fra-1, egr-1, and cyclinD1 genes, as well as the effects of sodium butyrate, an inhibitor of histone deacetylase activity, on transcription of these genes in normal REF cells and transformants E1A + ras.