NADH supplementation decreases pinacidil-primed I K ATP in ventricular cardiomyocytes by increasing intracellular ATP

1 The aim of this study was to investigate the effect of nicotinamide-adenine dinucleotide (NADH) supplementation on the metabolic condition of isolated guinea-pig ventricular cardiomyocytes. The pinacidil-primed ATP-dependent potassium current I(K(ATP)) was used as an indicator of subsarcolemmal AT...

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Bibliographic Details
Published in:British journal of pharmacology 2003-06, Vol.139 (4), p.749-754
Main Authors: Pelzmann, Brigitte, Hallström, Seth, Schaffer, Peter, Lang, Petra, Nadlinger, Karl, Birkmayer, George D, Vrecko, Karoline, Reibnegger, Gilbert, Koidl, Bernd
Format: Article
Language:English
Subjects:
Action Potentials
Adenosine Triphosphate - physiology
Animals
Dose-Response Relationship, Drug
Glyburide - pharmacokinetics
Guinea Pigs
Heart Ventricles
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
NAD - pharmacokinetics
NAD - physiology
Patch-Clamp Techniques
Pinacidil - administration & dosage
Pinacidil - antagonists & inhibitors
Pinacidil - pharmacokinetics
Potassium Channels - drug effects
Potassium Channels - physiology
Sarcolemma - chemistry
Sarcolemma - drug effects
Sarcolemma - enzymology
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Summary:1 The aim of this study was to investigate the effect of nicotinamide-adenine dinucleotide (NADH) supplementation on the metabolic condition of isolated guinea-pig ventricular cardiomyocytes. The pinacidil-primed ATP-dependent potassium current I(K(ATP)) was used as an indicator of subsarcolemmal ATP concentration and intracellular adenine nucleotide contents were measured. 2 Membrane currents were studied using the patch-clamp technique in the whole-cell recording mode at 36-37 degrees C. Adenine nucleotides were determined by HPLC. 3 Under physiological conditions (4.3 mM ATP in the pipette solution, ATP(i)) I(K(ATP)) did not contribute to basal electrical activity. 4 The ATP-dependent potassium (K((ATP))) channel opener pinacidil activated I(K(ATP)) dependent on [ATP](i) showing a significantly more pronounced activation at lower (1 mM) [ATP](i). 5 Supplementation of cardiomyocytes with 300 micro g ml(-1) NADH (4-6 h) resulted in a significantly reduced I(K(ATP)) activation by pinacidil compared to control cells. The current density was 13.8+/-3.78 (n=6) versus 28.9+/-3.38 pA pF(-1) (n=19; P
ISSN:0007-1188