Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cyt b559 and CP47/D1/D2/cyt b559 complexes)

• Published in: FEBS letters 1992-12, Vol.314 (3), p.271-274
• Main Authors: Moskalenko, Andrey A., Barbato, Roberto, Giacometti, Giorgio M.
• Format: Article
• Language: English
• Subjects: 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone; Bacterial Proteins - chemistry; Chl; chlorophyll; chlorophyll protein; Cross-Linking Reagents; Crosslinker; cyt; cytochrome; Cytochrome b Group - chemistry; DDIMB; disuccinimidyl tartarate; dithiobis(succinimidylpropionate); DSP; DST; EDC; EGS; Electrophoresis, Gel, Two-Dimensional; ethylenglycolbis(succinimidyl succinate); Immunoblotting; Light-Harvesting Protein Complexes; N-hydroxysulfosuccinimide; Nearest neighbour; NHS; Pheo; pheophytin; Photobleaching; Photocrosslinking; Photosynthetic Reaction Center Complex Proteins - chemistry; photosystem; Photosystem II; Photosystem II Protein Complex; Reaction center
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81487-7

The nearest neighbour relationships within the D1/D2/cyt b 559 complex (PSIIRC) and the CP47/D1/D2/cyt b 559 complex (RC-CP47) were investgated by using different length bifunctional crosslinking agents. The crosslinking products were identified by immunoblotting with polyclonal antibodies and by two-dimensional gel electrophoresis. Seven products (CP47/D2, D1/D2/α, D1/D2, D2/α, D1/α, α/αa, α/β) have been revealed in both complexes. The crosslinking of both complexes does not increase their photostability. The photocrosslinking products (D1/α and D2/α) appeared under illumination of complexes with light of high intensity.