Prognostic and metastatic value of phosphatase of regenerating liver-3 in invasive breast cancer

• Published in: Journal of cancer research and clinical oncology 2010-09, Vol.136 (9), p.1349-1357
• Main Authors: Hao, Ru-Tian, Zhang, Xiao-Hua, Pan, Yi-Fei, Liu, Hai-Guang, Xiang, You-Qun, Wan, Li, Wu, Xiu-Ling
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antineoplastic agents; Biological and medical sciences; Breast cancer; breast neoplasms; Breast Neoplasms - diagnosis; Breast Neoplasms - enzymology; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer Research; Female; Gene expression; Gynecology. Andrology. Obstetrics; Hematologic and hematopoietic diseases; Hematology; Humans; Immunohistochemistry; Internal Medicine; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymph node metastasis; Lymphatic Metastasis - pathology; Lymphatic system; Mammary gland diseases; Medical prognosis; Medical sciences; Medicine; Medicine & Public Health; Metastasis; Middle Aged; Neoplasm Invasiveness - diagnosis; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - metabolism; Oncology; Original Paper; Pharmacology. Drug treatments; PRL-3; Prognosis; Protein Tyrosine Phosphatases - biosynthesis; Protein Tyrosine Phosphatases - metabolism; Recurrence; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Survival Analysis; Tumors
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-010-0786-y

Purpose The aim of this study was to investigate the expression of the PRL-3 in human invasive breast cancer and to evaluate its clinical and prognostic significance. Its potential role in the invasive-metastatic properties of invasive breast cancer was also investigated. Methods Protein expression of PRL-3 was evaluated by immunohistochemistry for a consecutive series of 82 invasive human breast cancer tissues and 63 matched lymph node metastases, including PRL-3 mRNA expression analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) in malignant, nonmalignant breast tissue samples and lymph node metastases. We investigated the correlation of PRL-3 with clinicopathologic features, Overall and recurrence-free survival distribution curves were assessed using the Kaplan-Meier test and log-rank statistics, followed by Cox proportional hazards regression model. Results We found that 70.7% patients expressed a high level of PRL-3 protein in their tumors, and its over expression was positive correlated with lymph node metastasis (LNM) (P = 0.011). Moreover, The PRL-3 mRNA expression was significantly higher in malignant compared to benign breast tissue, while increased expression of PRL-3 mRNA was significantly associated with LNM (P = 0.002). Univariate analysis showed that the positive expression of PRL-3 was a poor risk prognostic factor (OS, P = 0.045; RFS, P = 0.034). Multivariate analysis using the Cox regression model indicated that high PRL-3 expression was an independent unfavorable prognostic factor for RFS. Conclusions These results strongly suggest that PRL-3 expression can indicate the potential role of LNM to some extent. Increasing the risk of tumor metastasis (OR = 3.889). Our results also imply that PRL-3 might be a novel molecular marker for predicting relapse of invasive breast cancer.