Production of lipopeptides in Bacillus sp. CS93 isolated from Pozol

Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. T...

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Bibliographic Details
Published in:FEMS microbiology letters 2010-03, Vol.304 (1), p.69-73
Main Authors: Moran, Stephen, Robertson, Keith, Paradisi, Francesca, Rai, Dilip K, Murphy, Cormac D
Format: Article
Language:English
Subjects:
Amplification
Anti-Bacterial Agents - metabolism
Antibacterial activity
Bacillus
Bacillus - genetics
Bacillus - growth & development
Bacillus - isolation & purification
Bacillus - metabolism
Bacillus subtilis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Culture Media
Deoxyribonucleic acid
DNA
Fermentation
Gene sequencing
Genes
Homology
Iturin A
Lipopeptides
Lipopeptides - biosynthesis
Liquid chromatography
Microbiology
Molecular ions
Multigene Family
Nucleotide sequence
Peptide synthase
Peptides, Cyclic - biosynthesis
Polymerase Chain Reaction
Primers
Surfactin
Zea mays - microbiology
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Summary:Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE were determined, but no gene that might code for a halogenating enzyme was detected either within the gene cluster or in the flanking sequences. Following further analysis of culture supernatants that were active against bacteria by liquid chromatography-MS, it was not possible to detect bacilysin/chlorotetaine. However, in methanolic fractions containing antibacterial activity, molecular ions characteristic of surfactins and fengycin were detectable by electrospray MS. Using primers complementary for conserved regions of nonribosomal peptide synthase, it was possible to amplify gene fragments that had a high degree of homology with known surfactin and fengycin biosynthetic genes. Thus, in addition to the known antimicrobial compounds, we have shown that this strain produces other bioactive lipopeptides, which might account for some of the medicinal properties of Pozol.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2009.01882.x