High Periconceptional Protein Intake Modifies Uterine and Embryonic Relationships Increasing Early Pregnancy Losses and Embryo Growth Retardation in Sheep

• Published in: Reproduction in domestic animals 2010-08, Vol.45 (4), p.723-728
• Main Authors: Meza-Herrera, CA, Ross, TT, Hallford, DM, Hawkins, DE, Gonzalez-Bulnes, A
• Format: Article
• Language: English
• Subjects: Abortion, Veterinary - etiology; Animal Feed - analysis; Animal reproduction; Animals; Biological and medical sciences; Diet - veterinary; Dietary Proteins - administration & dosage; Dietary Proteins - pharmacology; Dose-Response Relationship, Drug; Embryos; Female; Fetal Growth Retardation - chemically induced; Fetal Growth Retardation - veterinary; Fundamental and applied biological sciences. Psychology; Mammalian reproduction. General aspects; Pregnancy; Pregnancy, Animal; Proteins; Sheep; Sheep - embryology; Sheep - physiology; Uterus - drug effects; Uterus - physiology; Vertebrates: reproduction
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2009.01341.x

The effects of supplemented protein level (PL) during the periconceptional period and their interaction with body condition were evaluated in sheep. Multiparous Rambouillet ewes (n = 12) received two PL of rumen undegradable protein (UIP) during a 30-day pre-mating and 15-day post-mating period: low [LPL, 24% crude protein (CP), 14 g UIP and 36 g/CP animal/day] and high [HPL, 44% CP, 30 g UIP and 50 g/CP animal/day]. While ovulation rate (OR) did not differ between treatments (1.6 ± 0.5, mean ± SEM), a lower fertility rate, a decreased embryo number and a reduced uterine pH (UpH) was observed in the HPL group (p < 0.05), irrespective of BC. Luteal tissue weight, volume and progesterone secretion did not differ among treatments. Sheep with lower UpH also had lower conceptus weight (Cwt; p < 0.05, r = 0.65) and conceptuses with lower mass tended to secrete less INF-τ and IGF-1, and the correspondent endometrial explants had a higher basal PGF₂α release. Current study indicates that high protein diets during the periconceptional period in sheep modify uterine and embryonic relationships, increasing early pregnancy losses and inducing embryo growth retardation. Surviving embryos were affected by weight reductions, which could compromise later foetal growth and birth weight. Results evidence the key role of a balanced diet in reproductive success and indicate that the quality and nutrient composition of the maternal diet are essential for an adequate establishment of pregnancy, having paramount effects on the interplay of the embryo and the uterus.