Viability of human fibroblasts in coconut water as a storage medium

• Published in: International endodontic journal 2009-09, Vol.42 (9), p.827-830
• Main Authors: Moreira-Neto, J. J. S., Gondim, J. O., Raddi, M. S. G., Pansani, C. A.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; avulsion; Cell Survival - drug effects; Cells, Cultured; coconut water; Cocos; Dentistry; Fibroblasts - cytology; Fibroblasts - drug effects; human fibroblasts; Humans; Hydrogen-Ion Concentration; Isotonic Solutions; Milk; Organ Preservation Solutions; Osmolar Concentration; Plant Exudates - therapeutic use; Plant Preparations - therapeutic use; storage media; Tissue Preservation - methods; Tooth Avulsion
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2009.01591.x

Aim  To evaluate the effectiveness of a new storage medium for avulsed teeth, coconut water, in maintaining the viability of human fibroblasts. Methodology  Cell viability after different time periods was evaluated in the following storage media: coconut water, coconut water with sodium bicarbonate, milk, saline and still mineral water. Human fibroblasts were seeded in Eagle’s minimal essential medium (EMEM) supplemented with 7.5% foetal calf serum. After trypsinisation, 100 μL of culture medium containing approximately 104 cells mL−1 were collected and pipetted into the wells of 96‐well plates, which were incubated overnight in 5% CO2 and 95% air mixture at 37 °C. EMEM was then replaced by the storage media and the plates were incubated at 37 °C for 1, 2 and 4 h. Cell viability was determined using the neutral red assay. The proportions of viable cells after exposure to the storage media were analysed statistically by anova and the least significant difference (LSD) test (α = 5%). Results  Milk had the greatest capacity to maintain cell viability (P