Cardioprotection with forsythoside B in rat myocardial ischemia-reperfusion injury: Relation to inflammation response

• Published in: Phytomedicine (Stuttgart) 2010-07, Vol.17 (8), p.635-639
• Main Authors: Jiang, W.-L., Fu, F.-H., Xu, B.-M., Tian, J.-W., Zhu, H.-B., Jian-Hou
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Care and treatment; Complications and side effects; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Forsythoside B; Genetic aspects; Glycosides - pharmacology; Glycosides - therapeutic use; Health aspects; Heart - drug effects; Hemodynamics; Hemodynamics - drug effects; High-mobility group box 1; Inflammation - metabolism; Inflammation - prevention & control; Inflammation Mediators - metabolism; Inflammation response; Interleukin-6 - blood; Ischemia-reperfusion; Lamiaceae - chemistry; Leukocytes - drug effects; Leukocytes - metabolism; Male; Malondialdehyde - metabolism; Medicine, Chinese; Myocardial Infarction - metabolism; Myocardial Infarction - prevention & control; Myocardial ischemia; Myocardial Reperfusion Injury - drug therapy; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocardium - metabolism; Myocardium - pathology; Peroxidase - metabolism; Physiological aspects; Phytotherapy; Rats; Rats, Sprague-Dawley; Reperfusion injury; Risk factors; Transcription factors; Troponin T; Troponin T - blood; Tumor Necrosis Factor-alpha - blood
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2009.10.017

The present study was undertaken to examine the effect of forsythoside B (FB) on rat myocardial ischemia-reperfusion (I/R) model and elucidate the potential mechanism. Left ventricular systolic pressure (LVSP) and ±dp/dt max were detected. Blood samples were collected to determine serum levels of troponin T (Tn-T), TNF-α and IL-6. Hearts were harvested to assess histopathological change and infarct size, determine content of MDA, myeloperoxidase (MPO), SOD and GPx activities, analyze expression of high-mobility group box 1 (HMGB1), phosphor-IκB–α and phosphor-nuclear factor kappaB (NF-κB) in ischemic myocardial tissue by Western blot. Compared with control group, rats treatment with FB showed a significant recovery in myocardial function with improvement of LVSP and ±dp/dt max. The myocardial infarct volume, serum levels of Tn-T, TNF-α and IL-6, content of MDA and MPO activity in myocardial tissue were all reduced, protein expression of HMGB1, phosphor-IκB–α and phosphor-NF-κB were down-regulated, while attenuated the decrease of SOD and GPx activities. Besides, the infiltration of polymorph nuclear leukocytes (PMNs) and histopathological damages in myocardium were decreased in FB treated groups. These findings suggested that FB rescued cardiac function from I/R injury by limiting inflammation response and its antioxidant properties.