Preparation, Characterization, and Antiproliferative Activities of the Se-Containing Polysaccharide SeGLP-2B-1 from Se-Enriched Ganoderma lucidum

• Published in: Journal of agricultural and food chemistry 2009-09, Vol.57 (17), p.7737-7742
• Main Authors: Shang, Dejing, Zhang, Jianing, Wen, Lei, Li, Yang, Cui, Qiao
• Format: Article
• Language: English
• Subjects: anticarcinogenic activity; Antineoplastic Agents - pharmacology; Bioactive Constituents; Biological and medical sciences; Breast Neoplasms; Carcinoma, Hepatocellular; cell culture; Cell Cycle - drug effects; Cell Division - drug effects; Cell Line, Tumor; cell lines; cell proliferation; chemical composition; Chemical Phenomena; cytotoxicity; dietary mineral supplements; dietary minerals; Dietary Supplements; Female; Food industries; Fundamental and applied biological sciences. Psychology; galactose; Ganoderma - chemistry; Ganoderma lucidum; glucose; HeLa Cells; human diseases; human nutrition; Humans; K562 Cells; Liver Neoplasms; Mycelium - chemistry; neoplasms; Organometallic Compounds - chemistry; Organometallic Compounds - isolation & purification; Organometallic Compounds - pharmacology; polysaccharides; Polysaccharides - analysis; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; rhamnose; selenium; Selenium - administration & dosage; Selenium - analysis; xylose
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf9019344

Inorganic selenite can be transformed into organic forms and bind to proteins and polysaccharides in Se-enriched submerged Ganoderma lucidum cultures. In the present study, a novel Se-containing polysaccharide, SeGLP-2B-1, was purified from the Se-enriched mycelia of G. lucidum and the antiproliferative activities against six human cancer cell lines were investigated. The Se content of SeGLP-2B-1 was 186.7 μg/g, which was 150-fold larger than that of the regular polysaccharide GLP-2B-1 (1.3 μg/g). SeGLP-2B-1 (1.06 × 106 Da) was composed of glucose, rhamnose, xylose, and galactose with a molar ratio of 1.000:0.652:0.443:0.227. SeGLP-2B-1 exhibited an approximately 10-fold stronger antiproliferative activity against six human cancer cell lines as compared to GLP-2B-1. Thus, Se is believed to play an important role in increasing the antiproliferative property of SeGLP-2B-1. These findings indicate that SeGLP-2B-1 may serve as a dietary Se supplement.