Reduced expression and desensitization of adenosine A1 receptor/adenylyl cyclase pathway after chronic (−)N6 -phenylisopropyladenosine intake during pregnancy

• Published in: Neuroscience 2009-10, Vol.163 (2), p.524-532
• Main Authors: León, D.A, Castillo, C.A, Albasanz, J.L, Martín, M
• Format: Article
• Language: English
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; Adenosine A1 Receptor Agonists; Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - metabolism; Animals; Biological and medical sciences; Brain - drug effects; Brain - embryology; Brain - metabolism; Cell Membrane - drug effects; Cell Membrane - metabolism; Central Nervous System Agents - pharmacology; Colforsin - pharmacology; Female; Fundamental and applied biological sciences. Psychology; GTP-Binding Protein alpha Subunit, Gi2 - metabolism; GTP-Binding Protein alpha Subunits, Gi-Go - metabolism; Neurology; Phenylisopropyladenosine - pharmacology; Pregnancy; Rats; Rats, Wistar; Receptor, Adenosine A1 - metabolism; RNA, Messenger - metabolism; Signal Transduction - drug effects; Vertebrates: nervous system and sense organs
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2009.06.050

Abstract Little is known about the G protein–coupled receptor desensitization process during pregnancy. Wistar pregnant rats were treated with (−)N6 -phenylisopropyladenosine (R-PIA), an adenosine A1 receptor (A1 R) agonist, in their drinking water during pregnancy, and the effect on A1 R/adenylyl cyclase system was studied in both maternal and fetal brain. In maternal brain, binding assays revealed a significant decrease in total receptor numbers in plasma membranes (27%, P