Melanin-concentrating hormone receptor 2 affects 3T3-L1 preadipocyte differentiation

To investigate the influence of melanin-concentrating hormone receptor 2 (MCHR2) on preadipocyte proliferation and differentiation, a 3T3-L1-MCHR2 cell line stably expressing human MCHR2 was established. The expression level and biological activity of MCHR2 were evaluated by immunofluorescence and r...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular endocrinology 2009-11, Vol.311 (1), p.11-17
Main Authors: Yang, Junxia, Yuan, Chengfu, Wei, Lili, Yi, Faping, Song, Fangzou
Format: Article
Language:English
Subjects:
3T3-L1 Cells
3T3-L1 preadipocyte
Adipocytes - cytology
Adipocytes - metabolism
Animals
Biomarkers - metabolism
Cell Cycle
Cell Differentiation
Cell Membrane - metabolism
Cell Proliferation
Cell Shape
Differentiation
Fluorescent Antibody Technique
Humans
Lipid Metabolism
MCHR2
Mice
Obesity
Proliferation
Protein Binding
Receptors, G-Protein-Coupled - metabolism
Receptors, Pituitary Hormone - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate the influence of melanin-concentrating hormone receptor 2 (MCHR2) on preadipocyte proliferation and differentiation, a 3T3-L1-MCHR2 cell line stably expressing human MCHR2 was established. The expression level and biological activity of MCHR2 were evaluated by immunofluorescence and radioligand binding assays. A 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell cycle analysis showed that MCH did not affect 3T3-L1-MCHR2 and 3T3-L1-mock cell proliferation. MCH accelerated preadipocyte differentiation of the two cell lines when they were administered various doses of standard adipogenic inducers. The rate and degree of differentiation of the 3T3-L1-MCHR2 cells were higher than those of the 3T3-L1-mock cells. The expression levels of adipocyte-specific marker genes, including peroxisome proliferator-activated receptorγ2 (PPARγ2), CCAAT enhancer-binding proteinα (C/EBPα), adipocyte-specific fatty acid-binding protein (aP 2) and leptin were significantly up-regulated, especially in 3T3-L1-MCHR2 cells. These findings suggest that MCHR2 positively mediates the regulation of MCH during preadipocyte differentiation and is involved in energy balance regulation without affecting preadipocyte proliferation.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2009.03.023