Ultraviolet Photodissociation: Developments towards Applications for Mass-Spectrometry-Based Proteomics

Unraveling of all of the information contained in proteomes poses a tremendous chemical challenge, which is balanced by the promise of potentially transformational knowledge. Mass spectrometry offers an unprecedented arsenal of tools for diverse proteomic investigations. Recently, it was demonstrate...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2009-09, Vol.48 (39), p.7130-7137
Main Authors: Ly, Tony, Julian, Ryan R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Lasers
mass spectrometry
Mass Spectrometry - methods
Molecular Structure
Peptide Fragments - chemistry
Peptides - chemistry
Phosphorylation
Photophosphorylation
Proteomics - methods
radicals
sequence determination
Spectrophotometry, Ultraviolet - methods
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Summary:Unraveling of all of the information contained in proteomes poses a tremendous chemical challenge, which is balanced by the promise of potentially transformational knowledge. Mass spectrometry offers an unprecedented arsenal of tools for diverse proteomic investigations. Recently, it was demonstrated that ultraviolet light can be utilized to initiate unique and potentially useful fragmentations in peptides and proteins. Either nonspecific dissociation or highly specific dissociation at engineered chromophoric sites is possible following photon absorption. The level of specificity and control over fragmentation in these experiments is greater than with other fragmentation methods. Novel techniques made possible by this technology are poised to make substantial contributions to the field of proteomics.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200900613