A DNA biosensor based on the detection of doxorubicin-conjugated Ag nanoparticle labels using solid-state voltammetry

This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5′-CCA AGC AAC AGA CTC AAA-3′. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementa...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-10, Vol.25 (2), p.282-287
Main Authors: Ting, Boon Ping, Zhang, Jie, Gao, Zhiqiang, Ying, Jackie Y.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
DNA biosensor
DNA, Viral - analysis
DNA, Viral - genetics
Doxorubicin
Doxorubicin - chemistry
Electrochemistry
Electrochemistry - instrumentation
Electrodes
Equipment Design
Equipment Failure Analysis
Flu virus H5N1
Fundamental and applied biological sciences. Psychology
Immunoassay
Influenza A Virus, H5N1 Subtype - genetics
Methods. Procedures. Technologies
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Oligonucleotide Array Sequence Analysis - instrumentation
Semiconductors
Silver - chemistry
Silver nanoparticle labels
Solid-state voltammetry
Staining and Labeling
Transducers
Various methods and equipments
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Summary:This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5′-CCA AGC AAC AGA CTC AAA-3′. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementary to the target DNA. This modified Au electrode was incubated in a buffer solution containing the target DNA to form double-stranded DNA (ds-DNA) through hybridization. The ds-DNA on the electrode surface was then labeled with silver nanoparticles conjugated with a well-known DNA intercalator, doxorubicin. By performing cyclic voltammetry in an aqueous KCl solution (0.3M), the silver nanoparticle labels were detected as a result of the highly characteristic solid-state Ag/AgCl redox process. The signal obtained was subsequently used to quantify the amount of DNA. A detection limit of 1pM has been achieved with this new DNA biosensor.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.07.005