Differential cytokine expression by human dendritic cells in response to different Porphyromonas gingivalis capsular serotypes

• Published in: Journal of clinical periodontology 2009-10, Vol.36 (10), p.823-829
• Main Authors: Vernal, Rolando, León, Rubén, Silva, Augusto, Van Winkelhoff, Arie J., Garcia-Sanz, Jose A., Sanz, Mariano
• Format: Article
• Language: English
• Subjects: Animal models; Antigenicity; Antigens, Bacterial - immunology; Bacterial Capsules - classification; Bacterial Capsules - immunology; Biological and medical sciences; Capsular polysaccharides; capsular serotypes; Cells, Cultured; Cytokines; Cytokines - analysis; Cytokines - immunology; Dendritic cells; Dendritic Cells - immunology; Dentistry; Facial bones, jaws, teeth, parodontium: diseases, semeiology; g-Interferon; Gene expression; Gram-negative bacteria; Helper cells; Humans; Interferon-gamma - analysis; Interleukin 1; Interleukin 10; Interleukin 12; Interleukin 13; Interleukin 2; Interleukin 5; Interleukin 6; Interleukin-10 - analysis; Interleukin-12 - analysis; Interleukin-12 Subunit p35 - analysis; Interleukin-12 Subunit p40 - analysis; Interleukin-13 - analysis; Interleukin-1beta - analysis; Interleukin-2 - analysis; Interleukin-5 - analysis; Interleukin-6 - analysis; Lymphocytes T; Lymphotoxin-alpha - analysis; Medical sciences; Multiplicity of infection; Non tumoral diseases; Otorhinolaryngology. Stomatology; Pathogenicity; Polysaccharides, Bacterial - immunology; Porphyromonas gingivalis; Porphyromonas gingivalis - classification; Porphyromonas gingivalis - immunology; Porphyromonas gingivalis - pathogenicity; Serotypes; Serotyping; Th1 Cells - immunology; Tumor necrosis factor; Tumor Necrosis Factor-alpha - analysis; Virulence
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2009.01462.x

Aim: Capsular polysaccharides play an important role in the virulence of Gram‐positive and Gram‐negative bacteria. In Porphyromonas gingivalis, six serotypes have been described based on capsular antigenicity and its pathogenicity has been correlated both in vitro and in animal models. This study aimed to investigate the differential response of human dendritic cells (DCs) when stimulated with different P. gingivalis capsular serotypes. Materials and Methods: Using different multiplicity of infection (MOI) of the encapsulated strains K1–K6 and the non‐encapsulated K− strain of P. gingivalis, the mRNA expression levels for interleukin (IL)‐1β, IL‐2, IL‐5, IL‐6, IL‐10, IL‐12, IL‐13, interferon (IFN)‐γ, tumour necrosis factor (TNF)‐α, and TNF‐β in stimulated DCs were quantified by real‐time reverse transcription‐polymerase chain reaction. Results: All P. gingivalis capsular serotypes induced a T‐helper type 1 (Th1) pattern of cytokine expression. K1‐ and K2‐stimulated DCs expressed higher levels of IL‐1β, IL‐6, IL‐12p35, IL‐12p40, and IFN‐γ and at lower MOI than DCs stimulated with the other strains. Conclusions: These results demonstrate a differential potential of P. gingivalis capsular serotypes to induce DC responses and a higher capacity of strains K1 W83 and K2 HG184 than other K serotypes to trigger cytokine expression.