Differential remodeling of mono- and trimethylated H3K27 during porcine embryo development

Histone methylation plays an important role in regulating chromatin structure and gene expression. Methylation of the lysine residue 27 of histone H3 (H3K27) is an epigenetic mark that is closely linked with transcriptional repression; global patterns of H3K27 methylation undergo dramatic changes du...

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Bibliographic Details
Published in:Molecular reproduction and development 2009-11, Vol.76 (11), p.1033-1042
Main Authors: Park, Ki-Eun, Magnani, Luca, Cabot, Ryan A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastocyst - metabolism
Cell Nucleus - metabolism
Chromatin Assembly and Disassembly
DNA Methylation
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Embryo, Mammalian - embryology
Embryology: invertebrates and vertebrates. Teratology
Embryonic Development
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Histones - metabolism
Oocytes - metabolism
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Repressor Proteins - genetics
Repressor Proteins - metabolism
Swine
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Histone methylation plays an important role in regulating chromatin structure and gene expression. Methylation of the lysine residue 27 of histone H3 (H3K27) is an epigenetic mark that is closely linked with transcriptional repression; global patterns of H3K27 methylation undergo dramatic changes during cleavage development in the mouse. The aim of this study was to characterize the H3K27 methylation pattern in cleavage stage porcine embryos obtained either by in vivo or in vitro fertilization or parthenogenetic activation and to determine the expression patterns of EED, EZH2, and SUZ12 (regulators of H3K27 methylation). We found that monomethylated H3K27 was detectable in the nuclei of oocytes and pronuclear, 2‐cell, 4‐cell, 8‐cell, and blastocyst stage embryos. Trimethylated H3K27 was detectable in the nuclei of GV stage oocytes, the chromosome of MII stage oocytes and a single pronucleus of the pronuclear stage embryos produced by fertilization; the signals were faint or absent in nuclei of two‐cell through blastocyst stage embryos. In addition, EED transcripts were increased from the four‐cell stage (P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.21061